Effect of superoxide dismutase plus catalase on Ca2+ transport in ischemic and reperfused skeletal muscle,
dc.contributor.author | Lee, Kevin R. | en_US |
dc.contributor.author | Cronenwett, Jack L. | en_US |
dc.contributor.author | Shlafer, Marshal | en_US |
dc.contributor.author | Corpron, Cynthia | en_US |
dc.contributor.author | Zelenock, Gerald B. | en_US |
dc.date.accessioned | 2006-04-07T19:58:49Z | |
dc.date.available | 2006-04-07T19:58:49Z | |
dc.date.issued | 1987-01 | en_US |
dc.identifier.citation | Lee, Kevin R., Cronenwett, Jack L., Shlafer, Marshal, Corpron, Cynthia, Zelenock, Gerald B. (1987/01)."Effect of superoxide dismutase plus catalase on Ca2+ transport in ischemic and reperfused skeletal muscle,." Journal of Surgical Research 42(1): 24-32. <http://hdl.handle.net/2027.42/26853> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WM6-4BNDXY8-VB/2/5d4525024ddb12d661f685c8f30471f4 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26853 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3807351&dopt=citation | en_US |
dc.description.abstract | Cytotoxic oxygen metabolites may contribute to skeletal muscle damage associated with ischemia and reperfusion. This study utilized a rat hindlimb ischemia model to investigate the effect of pretreatment with oxygen free radical scavengers superoxide dismutase (SOD) and catalase (CAT) on skeletal muscle Ca2+ uptake by sarcoplasmic reticulum (SR) in limbs subjected to periods of ischemia and reperfusion. SOD and CAT were conjugated to polyethylene glycol to prolong their half lives. Anesthetized rats (ca. 350 g) received an iv injection of either conjugated SOD (2 mg/kg) plus CAT (3.5 mg/kg) (n = 6, Treated Group) or 0.9 saline (4 ml/kg) (n = 6, Control Group) 5 min before unilateral hindlimb tourniquet ischemia of 3 hr duration. After 19 hr of reperfusion, muscle from each lower leg was excised and homogenized. Skeletal muscle SR was isolated by differential centrifugation. ATP-dependent Ca2+ uptake by the SR was then measured with dual wavelength spectrophotometry and used as an index of muscle function. Pretreatment with SOD and CAT maintained higher rates of Ca2+ uptake by SR of skeletal muscle from postischemic reperfused limbs (Treated Group 2.29 +/- 0.21 vs Control Group, 1.61 +/- 0.06 [mu]mole Ca2+/mg protein/min). These results implicate cytotoxic oxygen metabolites in the pathogenesis of ischemic reperfusion skeletal muscle injury. | en_US |
dc.format.extent | 722917 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Effect of superoxide dismutase plus catalase on Ca2+ transport in ischemic and reperfused skeletal muscle, | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Surgery and Anesthesiology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA; Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA. | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Department of Surgery, Dartmouth-Hitchcock Medical Center, Hanover, New Hampshire 03756, USA | en_US |
dc.contributor.affiliationother | Department of Surgery, Dartmouth-Hitchcock Medical Center, Hanover, New Hampshire 03756, USA | en_US |
dc.identifier.pmid | 3807351 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26853/1/0000417.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0022-4804(87)90060-6 | en_US |
dc.identifier.source | Journal of Surgical Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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