Continuous-flow enzymatic determination of creatinine with improved on-line removal of endogenous ammonia
dc.contributor.author | Collison, M. E. | en_US |
dc.contributor.author | Meyerhoff, Mark E. | en_US |
dc.date.accessioned | 2006-04-07T19:59:18Z | |
dc.date.available | 2006-04-07T19:59:18Z | |
dc.date.issued | 1987 | en_US |
dc.identifier.citation | Collison, M. E., Meyerhoff, M. E. (1987)."Continuous-flow enzymatic determination of creatinine with improved on-line removal of endogenous ammonia." Analytica Chimica Acta 200(): 61-72. <http://hdl.handle.net/2027.42/26866> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TF4-44Y3X2T-PV/2/080a097fc6cdb0de3be0d86e3537e0c5 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/26866 | |
dc.description.abstract | A new continuous-flow automated enzymatic method suitable for the direct determination of creatinine in physiological samples is described. The proposed system utilizes an on-line gas predialysis unit in conjuction with a flow-through enzyme reactor coil and a potentiometric ammonia detector. The enzyme reactor contains immobilized creatinine iminohydrolase (EC 3.5.4.21) which converts creatinine to ammonia and N-methylhydantoin. Ammonia liberated from this reaction is detected downstream with the membrane electrode-based detector. The novel gas predialysis unit effectively removes>99.8% of endogenous ammonia (up to 1 mM) present in the sample. Thus, final peak potentials recorded by the electrode detector are directly proportional to the logarithm of creatinine concentrations present. The method is shown to be precise (<3%), selective, and capable of accurately determining creatinine in serum and urine samples containing abnormally high endogenous ammonia levels. Determinations of creatinine in serum samples (n = 30) using this new method correlate well with an existing Technicon AutoAnalyzer colorimetric method (r = 0.996). | en_US |
dc.format.extent | 799645 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Continuous-flow enzymatic determination of creatinine with improved on-line removal of endogenous ammonia | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 U.S.A. | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 U.S.A. | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/26866/1/0000431.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0003-2670(00)83758-7 | en_US |
dc.identifier.source | Analytica Chimica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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