"Self-screening" of rhodopsin in rod outer segments
dc.contributor.author | Alpern, Mathew | en_US |
dc.contributor.author | Fulton, Anne B. | en_US |
dc.contributor.author | Baker, Barbara N. | en_US |
dc.date.accessioned | 2006-04-07T20:04:51Z | |
dc.date.available | 2006-04-07T20:04:51Z | |
dc.date.issued | 1987 | en_US |
dc.identifier.citation | Alpern, Mathew, Fulton, Anne B., Baker, Barbara N. (1987).""Self-screening" of rhodopsin in rod outer segments." Vision Research 27(9): 1459-1470. <http://hdl.handle.net/2027.42/27007> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T0W-484M7N2-1B7/2/30b8e2ade80a8ed136e5a3de5ef689ce | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27007 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3445480&dopt=citation | en_US |
dc.description.abstract | Microspectrophotometry (MSP) shows rhodopsin highly concentrated (about 3.0mmol/l) in rod outer segments (ROS). Calculation of the in vivo absorption spectrum of human rhodopsin from such data reveals a striking failure to agree with the action spectrum of human rod vision. Agreement is good between the spectral distribution of absorption coefficients and the action spectrum, but the "concentration-broadening" (or "self-screening") introduced by the high end on absorbance at this concentration results in a misfit among the largest in the 93 years comparisons of this kind have been made! To deal with this anomaly, it has been suggested that "concentration-broadening" is inappropriate for rhodopsin in rod vision. This proposal was tested by comparing rod action spectra of 15-day-old and adult rats, since the lengths of ROS increase by a factor of about two in maturation. Three lines of evidence are inconsistent with it. Although the conundrum remains unexplained, it cannot be dismissed by supposing "self-screening" inappropriate for night vision. | en_US |
dc.format.extent | 1244623 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | "Self-screening" of rhodopsin in rod outer segments | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Ophthalmology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Vision Research Laboratory, W.K. Kellogg Eye Center, University of Michigan, Ann Arbor, MI 48105, U.S.A. | en_US |
dc.contributor.affiliationother | Ophthalmology Department, Children's Hospital Medical Center, Boston, MA 02115, U.S.A. | en_US |
dc.contributor.affiliationother | Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, U.S.A. | en_US |
dc.identifier.pmid | 3445480 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27007/1/0000574.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0042-6989(87)90155-6 | en_US |
dc.identifier.source | Vision Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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