Cloning and sequencing of cDNA for the rat plasminogen activator inhibitor-1
dc.contributor.author | Zeheb, Ron | en_US |
dc.contributor.author | Gelehrter, Thomas D. | en_US |
dc.date.accessioned | 2006-04-07T20:06:22Z | |
dc.date.available | 2006-04-07T20:06:22Z | |
dc.date.issued | 1988-12-20 | en_US |
dc.identifier.citation | Zeheb, Ron, Gelehrter, Thomas D. (1988/12/20)."Cloning and sequencing of cDNA for the rat plasminogen activator inhibitor-1." Gene 73(2): 459-468. <http://hdl.handle.net/2027.42/27017> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T39-47PH5RP-X3/2/c776a42d065b6206a68143a5add563fb | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27017 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3149611&dopt=citation | en_US |
dc.description.abstract | A cDNA encoding rat plasminogen activator-inhibitor (PAI-1) has been isolated from an HTC rat hepatoma cell cDNA library constructed in phage [lambda]gt10. The cDNA contains 118 bp of 5'-untranslated sequence, 1206 bp encoding a 402-amino acid (aa) protein and 1747 bp of 3'-untranslated sequence. The protein-coding sequence and the derived amino acid sequence share 82% and 81% identity, respectively, with human PAI-1 cDNA and protein. The rat cDNA encodes a preprotein with a 23-aa leader peptide and a predicted N-terminal serine for the mature protein. Three of four potential N-glycosylation acceptor sites as well as the active site of rat PAI-1 are identical to the human protein. The 3'-untranslated region contains a number of unusual regions, including 80 bp of tandemly repeated GpA dinucleotides, a 115-bp stretch which shares greater than 90% sequence identity with a region within the 3'-untranslated cDNA of human PAI-1, and two 70-bp stretches of highly T-rich sequence located close to the 3'-terminus of the cDNA. | en_US |
dc.format.extent | 1248220 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Cloning and sequencing of cDNA for the rat plasminogen activator inhibitor-1 | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-0618, U.S.A. Tel.(313)763-3460 | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-0618, U.S.A. Tel.(313)763-3460 | en_US |
dc.identifier.pmid | 3149611 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27017/1/0000003.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0378-1119(88)90510-0 | en_US |
dc.identifier.source | Gene | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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