Site-specific mutagenesis in Legionella pneumophila by allelic exchange using counterselectable ColE1 vectors
dc.contributor.author | Cianciotto, Nicholas P. | en_US |
dc.contributor.author | Long, Robert | en_US |
dc.contributor.author | Eisenstein, Barry I. | en_US |
dc.contributor.author | Cary Engleberg, N. | en_US |
dc.date.accessioned | 2006-04-07T20:06:56Z | |
dc.date.available | 2006-04-07T20:06:56Z | |
dc.date.issued | 1988-12-01 | en_US |
dc.identifier.citation | Cianciotto, Nicholas P., Long, Robert, Eisenstein, Barry I., Cary Engleberg, N. (1988/12/01)."Site-specific mutagenesis in Legionella pneumophila by allelic exchange using counterselectable ColE1 vectors." FEMS Microbiology Letters 56(2): 203-207. <http://hdl.handle.net/2027.42/27030> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T2W-476W716-1VY/2/f0ac37ad71353d8df98f25fb1149a589 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27030 | |
dc.description.abstract | To study the molecular pathogenesis of infection by Legionella pneumophila, a technique of site-specific mutagenesis by allelic exchange was evaluated. To develop this system, we optimized conjugal DNA transfer by isolating a mutant that functions 1000-fold more efficiently as a recipient than the wild type strain, identified two counter-selectable markers, rpsL and sacB, that function in L. pneumophila, and constructed a counterselectable Co1E1 vector. Allelic exchange of a L. pneumophila chrosomal gene was achieved at a frequency of 10-5 per transconjugant. The allelic exchange procedure itself did not alter the ability of L. pneumophila to infect macrophages, indicating that the system can be used to study this aspect of virulence. | en_US |
dc.format.extent | 383835 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Site-specific mutagenesis in Legionella pneumophila by allelic exchange using counterselectable ColE1 vectors | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Microbiology and Immunology, The University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Microbiology and Immunology, The University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Internal Medicine, The University of Michigan, Ann Arbor, MI, U.S.A.; Departments of Microbiology and Immunology, The University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Departments of Microbiology and Immunology, The University of Michigan, Ann Arbor, MI, U.S.A.; Departments of Internal Medicine, The University of Michigan, Ann Arbor, MI, U.S.A.; Ann Arbor Veterans' Administration Hospital, Ann Arbor, MI, U.S.A. | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27030/1/0000018.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0378-1097(88)90191-7 | en_US |
dc.identifier.source | FEMS Microbiology Letters | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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