Effects of Rifampicin resistant rpoB mutations on antitermination and interaction with nusA in Escherichia coli
dc.contributor.author | Jin, Ding Jun | en_US |
dc.contributor.author | Cashel, Michael | en_US |
dc.contributor.author | Friedman, David I. | en_US |
dc.contributor.author | Nakamura, Yoshikazu | en_US |
dc.contributor.author | Walter, William A. | en_US |
dc.contributor.author | Gross, Carol A. | en_US |
dc.date.accessioned | 2006-04-07T20:08:27Z | |
dc.date.available | 2006-04-07T20:08:27Z | |
dc.date.issued | 1988-11-20 | en_US |
dc.identifier.citation | Jin, Ding Jun, Cashel, Michael, Friedman, David I., Nakamura, Yoshikazu, Walter, William A., Gross, Carol A. (1988/11/20)."Effects of Rifampicin resistant rpoB mutations on antitermination and interaction with nusA in Escherichia coli." Journal of Molecular Biology 204(2): 247-261. <http://hdl.handle.net/2027.42/27062> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WK7-4DN8YK9-KK/2/f33cf864b55a66e346631e783db1540a | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27062 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2464690&dopt=citation | en_US |
dc.description.abstract | Rifampicin resistant (Rifr mutations map in the rpoB gene encoding the [beta] subunit of Escherichia coli RNA polymerase. We have used our collection of 17 sequenced Rifr mutations to investigate the involvement of E. coli RNA polymerase in the antitermination systems enhancing expression of delayed early [lambda] genes or stable RNA. We have found that Rifr mutations affect both [lambda] N-mediated antitermination and the cellular antitermination system involved in synthesis of stable RNA. Because NusA is involved in antitermination and termination, we also investigated the interaction of NusA and RNA polymerase by determining whether Rifr mutations alter NusA-dependent termination or antitermination in cells with defective nusA alleles. We have shown that Rifr mutations can either enhance or suppress the phenotypes of defective nusA alleles. Most Rifr mutations alter the temperature range over which the nusA1 allele supports [lambda] N-mediated antitermination. In addition, a number of Rifr alleles restore termination to the nusA10(Cs) and the nusA11(Ts) mutants defective in this process. Our results indicate that the region of the rpoB gene defined by the Rifr mutations is involved in the antitermination process and affects the activity of the NusA protein directly or indirectly. | en_US |
dc.format.extent | 1626117 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Effects of Rifampicin resistant rpoB mutations on antitermination and interaction with nusA in Escherichia coli | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Bacteriology, University of Wisconsin, Madison, WI 53706, U.S.A. | en_US |
dc.contributor.affiliationother | Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda MD 20892, U.S.A. | en_US |
dc.contributor.affiliationother | The Institute of Medical Science, The University of Tokyo, P.O. Takanawa, Tokyo 108, Japan | en_US |
dc.contributor.affiliationother | Department of Bacteriology, University of Wisconsin, Madison, WI 53706, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Bacteriology, University of Wisconsin, Madison, WI 53706, U.S.A. | en_US |
dc.identifier.pmid | 2464690 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27062/1/0000052.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0022-2836(88)90573-6 | en_US |
dc.identifier.source | Journal of Molecular Biology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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