Stabilization of tetramethylbenzidine (TMB) reaction product at the electron microscopic level by ammonium molybdate
dc.contributor.author | Marfurt, Carl F. | en_US |
dc.contributor.author | Turner, Dennis F. | en_US |
dc.contributor.author | Adams, Catherine E. | en_US |
dc.date.accessioned | 2006-04-07T20:10:49Z | |
dc.date.available | 2006-04-07T20:10:49Z | |
dc.date.issued | 1988-10 | en_US |
dc.identifier.citation | Marfurt, Carl F., Turner, Dennis F., Adams, Catherine E. (1988/10)."Stabilization of tetramethylbenzidine (TMB) reaction product at the electron microscopic level by ammonium molybdate." Journal of Neuroscience Methods 25(3): 215-223. <http://hdl.handle.net/2027.42/27115> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T04-484M8FJ-6F/2/4883e4eb7e1dea19e09b5deb49b9a9d7 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27115 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3226148&dopt=citation | en_US |
dc.description.abstract | The ability to use the tetramethylbenzidine (TMB) method for studying neuronal connections at the electron microscopic level is often difficult because the conditions of osmification and dehydration used in processing the tissue may result in significant loss and/or decreased electron density of the reaction product. In the present study, we report that stabilization of TMB reaction product with 5% ammonium molybdate (AM) prior to osmificating the tissue results in the formation of TMB-AM crystals that are many times more electron dense and resistant to ethanol extraction than non-stabilized TMB crystals. The nature of the chemical interaction that underlies the stabilization of TMB by AM is uncertain, but it may involve the formation of an insoluble salt between molybdic ions and the TMB polymer. The use of this simple procedure increases the sensitivity of the TMB procedure at the electron microscopic level and may be used to label neuronal pathways in the peripheral and central nervous systems with equal success. | en_US |
dc.format.extent | 1387691 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Stabilization of tetramethylbenzidine (TMB) reaction product at the electron microscopic level by ammonium molybdate | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Oral Biology, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Anatomy, Northwest Center for Medical Education, Indiana University School of Medicine, Gary, IN 46408, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Anatomy, The Milton S. Hershey Medical Center, The Pennsylvania State University, Hershey, PA 17033, U.S.A. | en_US |
dc.identifier.pmid | 3226148 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27115/1/0000107.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0165-0270(88)90136-7 | en_US |
dc.identifier.source | Journal of Neuroscience Methods | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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