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Protein microinjection by protease permeabilization of fibroblasts
dc.contributor.authorLemons, Rosemary M.en_US
dc.contributor.authorForster, Susanen_US
dc.contributor.authorThoene, Jess G.en_US
dc.date.accessioned2006-04-07T20:16:39Z
dc.date.available2006-04-07T20:16:39Z
dc.date.issued1988-07en_US
dc.identifier.citationLemons, Rosemary, Forster, Susan, Thoene, Jess (1988/07)."Protein microinjection by protease permeabilization of fibroblasts." Analytical Biochemistry 172(1): 219-227. <http://hdl.handle.net/2027.42/27241>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6W9V-4DW2KKR-K6/2/007e9e67df66434ead8f9b3f793cc86fen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/27241
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2847580&dopt=citationen_US
dc.description.abstractExposure of cultured diploid fibroblasts to protease solutions induces a hyperpermeable state which permits entry of exogenous macromolecules directly into the cytosol. We have exploited this finding to devise a microinjection method whose chief advantages are simplicity and good retention of cell viability. Proteins successfully injected by this technique range from insulin to thyroglobulin. The amounts injected range from 4 x 105 to 5 x 106 molecules/cell.en_US
dc.format.extent2779862 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleProtein microinjection by protease permeabilization of fibroblastsen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, School of Medicine, Ann Arbor, Michigan 48109, USA; Department of Pediatrics, University of Michigan, School of Medicine, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, School of Medicine, Ann Arbor, Michigan 48109, USA; Department of Pediatrics, University of Michigan, School of Medicine, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Pediatrics, University of Michigan, School of Medicine, Ann Arbor, Michigan 48109, USA; Department of Biological Chemistry, University of Michigan, School of Medicine, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid2847580en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/27241/1/0000248.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0003-2697(88)90435-6en_US
dc.identifier.sourceAnalytical Biochemistryen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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