The carboxylmethylation of cerebral membrane-bound proteins increases with age
dc.contributor.author | Sellinger, Otto Z. | en_US |
dc.contributor.author | Kramer, Craig M. | en_US |
dc.contributor.author | Conger, Alicia | en_US |
dc.contributor.author | Duboff, Gregory S. | en_US |
dc.date.accessioned | 2006-04-07T20:19:18Z | |
dc.date.available | 2006-04-07T20:19:18Z | |
dc.date.issued | 1988-05 | en_US |
dc.identifier.citation | Sellinger, Otto Z., Kramer, Craig M., Conger, Alicia, Duboff, Gregory S. (1988/05)."The carboxylmethylation of cerebral membrane-bound proteins increases with age." Mechanisms of Ageing and Development 43(2): 161-173. <http://hdl.handle.net/2027.42/27307> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T31-47P9C16-4H/2/68be5903dfac21f6f744610362ee518b | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27307 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3398604&dopt=citation | en_US |
dc.description.abstract | Recently, we have characterized a membrane-bound (mb) component of brain protein carboxylmethyltransferase II (PCMT) which effectively carboxylmethylates endogenous mb methyl-accepting proteins (MAPs). (Neurochem. Int., 10 (1987) 155). We have also shown that exposing mb-MAPs to mild alkali leads to a marked increase in their recognition by PCMT. Since one of the likely consequences of the alkaline treatment appears to be the deamidation of selected protein-bound asparagines or aspartates, followed by the formation, in their place, of -or -isoaspartates, it is reasonable to assume that mb-MAPs constitute unique targets for the mb-PCMT because they contain such unnatural aspartate residues. Testing the relevance of this notion to the aging of cerebral mb-MAPs we focus in this report on age-related changes involving mb-MAPs. When two-or six-times washed (in 50 mM NaPO4 buffer, pH 6.5) 17 500 g, 30-min membranes or Percoll-gradient purified synaptic membranes were prepared from young (3-4 months) and old (11-12 months) rat brains and were incubated with 20 [mu]M [3H]methyl at pH 6.0, mb-MAP carboxyl[3H]methylation was significantly more intense in the old than in the young membranes, no additional increase being noted at 28-35 months. Mb-MAP carboxylmethylation increases were confirmed over a wide range of membrane protein concentrations and incubation times and are taken to reflect age-related modifications of the primary structure of susceptible mb-MAPs. To investigate these, we incubated young and old membranes, as well as their Lubrol-Px(1%) extracts (30 min, 0[deg]C), with 0.05 M NH4OH for 90 min at 37[deg]C, a treatment which left PCMT activity largely unaffected. Our findings reveal | en_US |
dc.format.extent | 797066 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | The carboxylmethylation of cerebral membrane-bound proteins increases with age | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Geriatrics | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Laboratory of Neurochemistry, Mental Health Research Institute, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Laboratory of Neurochemistry, Mental Health Research Institute, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Laboratory of Neurochemistry, Mental Health Research Institute, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Laboratory of Neurochemistry, Mental Health Research Institute, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 3398604 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27307/1/0000328.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0047-6374(88)90044-9 | en_US |
dc.identifier.source | Mechanisms of Ageing and Development | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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