A cellular model of endothelial cell ischemia
dc.contributor.author | Hinshaw, Daniel B. | en_US |
dc.contributor.author | Armstrong, Barbara C. | en_US |
dc.contributor.author | Beals, Theodore F. | en_US |
dc.contributor.author | Hyslop, Paul A. | en_US |
dc.date.accessioned | 2006-04-07T20:19:51Z | |
dc.date.available | 2006-04-07T20:19:51Z | |
dc.date.issued | 1988-05 | en_US |
dc.identifier.citation | Hinshaw, Daniel B., Armstrong, Barbara C., Beals, Theodore F., Hyslop, Paul A. (1988/05)."A cellular model of endothelial cell ischemia." Journal of Surgical Research 44(5): 527-537. <http://hdl.handle.net/2027.42/27321> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WM6-4BNF403-2K8/2/d9ae417659e209e602cd337d531a7e8b | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27321 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3374116&dopt=citation | en_US |
dc.description.abstract | Endothelial cell dysfunction in ischemia may cause increased capillary permeability. We examined the effect of failing ATP synthesis, a major consequence of ischemia, on microfilaments--important structural determinants of the endothelial cell. Glycolytic and mitochondrial ATP synthesis in bovine pulmonary artery endothelial cells was inhibited by glucose depletion and 650 picomole (pmole) oligomycin/[mu]g DNA, respectively. ATP levels were monitored with the luciferase-luciferin assay over a 2-hr time course followed by recovery for 1 hr after removal of the oligomycin and addition of 5.5 mM glucose. ATP levels fell to 83.6 +/- 63.8 pmole/[mu]g DNA (n = 11) by 30 min, 26.9 +/- 13.8 pmole/[mu]g DNA (n = 11) by 60 min, and 17.2 +/- 3.8 pmole/[mu]g DNA (n = 6) by 120 min, whereas control uninjured cells had 541.3 +/- 196.8 pmole/[mu]g DNA (n = 6) at 120 min. Fluorescence microscopy of microfilaments stained with rhodamine-phalloidin revealed progressive disassembly and shortening of the microfilaments in>90% of cells over 60 min which correlated with the fall in ATP. Ultrastructural examination revealed that side to side aggregation of microfilaments had occurred over the 120-min time course. Two hours of glucose depletion (305.5 +/- 130.8 pmole ATP/[mu]g DNA, n = 6) or oligomycin alone (480.0 +/- 90.1 pmole ATP/[mu]g DNA, n = 6) failed to produce the dramatic fall in ATP or the microfilament changes. During cell recovery, there was a rapid reassembly of microfilaments, detected by fluorescence microscopy, which was nearly complete in 85-90% of cells by 45-60 min. ATP levels increased significantly (P = 0.002) to 96.1 +/- 36.8 pmole/[mu]g DNA (n = 6) by 30 min. This model should provide insight into the pathogenesis and treatment of the capillary leak seen with ischemia. | en_US |
dc.format.extent | 1342516 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A cellular model of endothelial cell ischemia | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Surgery and Anesthesiology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Surgery, Medical College of Virginia, Richmond, Virginia 23298, USA b University of Michigan, Ann Arbor, Michigan 48105, USA | en_US |
dc.contributor.affiliationum | Departments of Surgery, Medical College of Virginia, Richmond, Virginia 23298, USA b University of Michigan, Ann Arbor, Michigan 48105, USA | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan, Ann Arbor, Michigan 48105, USA | en_US |
dc.contributor.affiliationother | Department of Immunology, Scripps Clinic and Research Foundation, La Jolla, California 92037, USA | en_US |
dc.identifier.pmid | 3374116 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27321/1/0000344.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0022-4804(88)90158-8 | en_US |
dc.identifier.source | Journal of Surgical Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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