Modulation of surface CD11/CD18 glycoproteins (Mo1, LFA-1, p150,95) by human mononuclear phagocytes
dc.contributor.author | Freyer, David R. | en_US |
dc.contributor.author | Morganroth, Melvin L. | en_US |
dc.contributor.author | Rogers, Clare E. | en_US |
dc.contributor.author | Arnaout, M. Amin | en_US |
dc.contributor.author | Todd, Robert F. III | en_US |
dc.date.accessioned | 2006-04-07T20:24:40Z | |
dc.date.available | 2006-04-07T20:24:40Z | |
dc.date.issued | 1988-02 | en_US |
dc.identifier.citation | Freyer, David R., Morganroth, Melvin L., Rogers, Clare E., Arnaout, M. Amin, Todd, III, Robert F. (1988/02)."Modulation of surface CD11/CD18 glycoproteins (Mo1, LFA-1, p150,95) by human mononuclear phagocytes." Clinical Immunology and Immunopathology 46(2): 272-283. <http://hdl.handle.net/2027.42/27416> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WCK-4CC97VY-12/2/daef4084458f2bfb8d014d2acb7b1e68 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27416 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3123109&dopt=citation | en_US |
dc.description.abstract | Mo1, LFA-1, and p150,95 are structurally related glycoproteins of the CD11/CD18 complex that are expressed on the membrane of human leukocytes. In the neutrophil, the surface expression of the CD11/CD18 complex is up-modulated (Mo1 > p150,95 >> LFA-1) by stimulatory factors that include calcium ionophore A23187, phorbol myristate acetate (PMA), and N--formyl--leucyl--phenylalanine (fMLP). Here, in an immunofluorescence analysis, we have examined CD11/CD18 glycoprotein expression by human monocytes, pulmonary alveolar macrophages (PAM, obtained by bronchoalveolar lavage), and breast milk macrophages (BMM) as compared to neutrophils before and after exposure to A23187 (1 [mu]M), fMLP (0.1 [mu]M), or PMA (0.1 [mu]g/ml) ft 37[deg]C. Unstimulated monocytes within unfractionated blood mononuclear cells kept at 4[deg]C (n = 13) expressed all three CD11/CD18 glycoproteins, and exposure to A23187 resulted in significant increases in the surface expression of Mol (median of 5.7-fold), LFA-1 (median of 2.1-fold), and p150,95 (median of 7.2-fold). Exposure to fMLP- or PMA-stimulated increases of lesser magnitude. CD11/CD18 expression by PAM (n = 9) was barely detectable and was unaffected by exposure to A23187. In contrast, BMM (n = 11) expressed all three CD11/CD18 glycoproteins (with considerable variability among specimens), but no increase was stimulated by A23187. These results demonstrate that monocytes, like neutrophils, have the capacity to respond to activating factors with an increase in CD11/CD18 glycoprotein expression; macrophage differentiation is accompanied by a loss (PAM) or retention (BMM) of CD11/CD18 expression that is unmodulated in response to activation. | en_US |
dc.format.extent | 899397 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Modulation of surface CD11/CD18 glycoproteins (Mo1, LFA-1, p150,95) by human mononuclear phagocytes | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics and Communicable Diseases, Section of Pediatric Hematology-Oncology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Division of Pulmonary Medicine, Department of Internal Medicine, Simpson Memorial Research Institue, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Division of Hematology/Oncology, Department of Internal Medicine, Simpson Memorial Research Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | Division of Hematology/Oncology, Department of Internal Medicine, Simpson Memorial Research Institute, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Department of Medicine, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02215, USA | en_US |
dc.identifier.pmid | 3123109 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27416/1/0000453.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0090-1229(88)90189-4 | en_US |
dc.identifier.source | Clinical Immunology and Immunopathology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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