Homogeneous enzyme-linked competitive binding assay for biotin based on the avidin-biotin interaction
dc.contributor.author | Daunert, Sylvia | en_US |
dc.contributor.author | Bachas, Leonidas G. | en_US |
dc.contributor.author | Meyerhoff, Mark E. | en_US |
dc.date.accessioned | 2006-04-07T20:26:55Z | |
dc.date.available | 2006-04-07T20:26:55Z | |
dc.date.issued | 1988 | en_US |
dc.identifier.citation | Daunert, Sylvia, Bachas, Leonidas G., Meyerhoff, Mark E. (1988)."Homogeneous enzyme-linked competitive binding assay for biotin based on the avidin-biotin interaction." Analytica Chimica Acta 208(): 43-52. <http://hdl.handle.net/2027.42/27454> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TF4-44Y3W62-J5/2/cf1c92185c80cf87876fd42394128a72 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27454 | |
dc.description.abstract | A homogeneous enzyme-linked competitive-binding assay for biotin with glucose-6-phosphate dehydrogenase (G6PDH), is described. This assay is based on the interaction between a G6PDH/biotin conjugate with avidin, a natural binder for biotin. In the absence of biotin in the assay mixture, this interaction results in 100% inhibition of the enzyme conjugate. In the presence of biotin, the enzymatic activity of the conjugate is regained in an amount related to the concentration of the vitamin in the sample. Extremely steep, gate-like dose/response curves, attributable to the relative binding affinities of avidin for biotin and the conjugate, are observed. The detection limits of the system vary with the amounts of avidin and enzyme/biotin conjugate used. The method is rapid and sensitive and is evaluated for the direct determination of biotin in vitamin tablets. | en_US |
dc.format.extent | 706527 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Homogeneous enzyme-linked competitive binding assay for biotin based on the avidin-biotin interaction | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055 U.S.A. | en_US |
dc.contributor.affiliationother | Department of Chemistry, University of Kentucky, Lexington, KY 40506-0055 U.S.A. | en_US |
dc.contributor.affiliationother | Department of Chemistry, University of Kentucky, Lexington, KY 40506-0055 U.S.A. | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27454/1/0000494.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0003-2670(00)80734-5 | en_US |
dc.identifier.source | Analytica Chimica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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