Systemic perfusion: a method of enhancing relative tumor uptake of radiolabeled monoclonal antibodies
dc.contributor.author | Wahl, Richard L. | en_US |
dc.contributor.author | Piko, Cynthia R. | en_US |
dc.contributor.author | Beers, Barbara A. | en_US |
dc.contributor.author | Geatti, Onelio | en_US |
dc.contributor.author | Johnson, Jon W. | en_US |
dc.contributor.author | Sherman, Phillip S. | en_US |
dc.date.accessioned | 2006-04-07T20:33:08Z | |
dc.date.available | 2006-04-07T20:33:08Z | |
dc.date.issued | 1988 | en_US |
dc.identifier.citation | Wahl, Richard L., Piko, Cynthia R., Beers, Barbara A., Geatti, Onelio, Johnson, Jon, Sherman, Phil (1988)."Systemic perfusion: a method of enhancing relative tumor uptake of radiolabeled monoclonal antibodies." International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology 15(6): 611-616. <http://hdl.handle.net/2027.42/27583> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B7GH9-4C00N8H-2B/2/888f7ed0fe14ae9b9c21328073e6a3ea | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27583 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3251896&dopt=citation | en_US |
dc.description.abstract | We evaluated the feasibility of systemic vascular perfusion with saline (mimicking plasmapheresis) as a method to enhance tumor-specific monoclonal antibody (MoAb) tumor/background ratios. Initially, groups of rats were injected intravenously (i.v.) with 131I-5G6.4 MoAb (murine IgG2aK reactive with ovarian carcinoma). These animal's radioactivity levels were determined by dose calibrator and they were imaged before and after perfusion which was conducted at 4 or 24 h post-antibody injection. Animals were sacrificed after perfusion, as were controls, and normal organ radioactivity levels determined. In addition, nude mice bearing HTB77 ovarian cancers subcutaneously were injected i.v. with 131I-5G6.4 MoAb and were imaged before and after systemic perfusion with saline 24 h post-5G6.4 injection. Perfusion in rats dropped whole-body 5G6.4 levels significantly at both perfusion times (P P P < 0.05). These studies show that (1) much background antibody radioactivity can be removed using whole-body perfusion with saline, (2) that the decline in whole body activity is larger with 4 than 24 h perfusion and (3) tumor imaging can be enhanced by this approach. This and similar approaches that increase relative tumor antibody uptake such as plasmapheresis may be useful in imaging and therapy with radiolabeled antibodies. | en_US |
dc.format.extent | 773711 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Systemic perfusion: a method of enhancing relative tumor uptake of radiolabeled monoclonal antibodies | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Physics | en_US |
dc.subject.hlbsecondlevel | Nuclear Engineering and Radiological Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109-0028, U.S.A. | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109-0028, U.S.A. | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109-0028, U.S.A. | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109-0028, U.S.A. | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109-0028, U.S.A. | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109-0028, U.S.A. | en_US |
dc.identifier.pmid | 3251896 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27583/1/0000627.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0883-2897(88)90051-7 | en_US |
dc.identifier.source | International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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