Molecular cloning and in vitro expression of a silent phenoxazinone synthase gene from Streptomyces lividans
dc.contributor.author | Madu, Anthony C. | en_US |
dc.contributor.author | Jones, George H. | en_US |
dc.date.accessioned | 2006-04-07T20:37:16Z | |
dc.date.available | 2006-04-07T20:37:16Z | |
dc.date.issued | 1989-12-14 | en_US |
dc.identifier.citation | Madu, Anthony C., Jones, George H. (1989/12/14)."Molecular cloning and in vitro expression of a silent phenoxazinone synthase gene from Streptomyces lividans." Gene 84(2): 287-294. <http://hdl.handle.net/2027.42/27637> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T39-47PH9TY-2HN/2/094e97cbd8c9b96bacc0a8528ff3afac | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27637 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2558968&dopt=citation | en_US |
dc.description.abstract | Phenoxazinone synthase (PHS) catalyzes a step in actinomycin D biosynthesis in Streptomyces antibioticus. Two sequences from Streptomyces lividans that hybridize to the phs gene of S. antibioticus have been cloned in Escherichia coli K-12 using the plasmid pBR322. Although there was some similarity in the restriction maps of the two cloned fragments, neither insert appeared to be a direct subset of the other nor of the S. antibioticus phs gene. In vitro expression studies, in a streptomycete coupled transcription-translation system, showed that a 3.98-kb SphI fragment encoded a PHS-related protein. These observations provide additional support for the existence of silent genes for antibiotic production in streptomycetes. | en_US |
dc.format.extent | 798981 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Molecular cloning and in vitro expression of a silent phenoxazinone synthase gene from Streptomyces lividans | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biology, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biology, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 2558968 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27637/1/0000013.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0378-1119(89)90502-7 | en_US |
dc.identifier.source | Gene | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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