Expression of human HPRT mRNA in brains of mice infected with a recombinant herpes simplex virus-1 vector
dc.contributor.author | Palella, Thomas D. | en_US |
dc.contributor.author | Hidaka, Yuji | en_US |
dc.contributor.author | Silverman, Larry J. | en_US |
dc.contributor.author | Levine, Myron | en_US |
dc.contributor.author | Glorioso, Joseph C. | en_US |
dc.contributor.author | Kelley, William N. | en_US |
dc.date.accessioned | 2006-04-07T20:43:49Z | |
dc.date.available | 2006-04-07T20:43:49Z | |
dc.date.issued | 1989-08-01 | en_US |
dc.identifier.citation | Palella, Thomas D., Hidaka, Yuji, Silverman, Larry J., Levine, Myron, Glorioso, Joseph, Kelley, William N. (1989/08/01)."Expression of human HPRT mRNA in brains of mice infected with a recombinant herpes simplex virus-1 vector." Gene 80(1): 137-144. <http://hdl.handle.net/2027.42/27810> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T39-47PH5TW-Y1/2/3a62a73bc2e12966c7a528cc3e434487 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27810 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2551779&dopt=citation | en_US |
dc.description.abstract | Complete deficiency of the purine salvage enzyme hypoxanthine-guanine phosphoribosyltransferase (HPRT) results in a devastating neurological disease, the Lesch-Nyhan syndrome. This disorder has been identified as a candidate for initial attempts at somatic cell gene therapy. We have previously reported the construction of a recombinant herpes simplex virus type 1 (HSV-1) vector containing human hprt cDNA sequences under the regulatory control of the viral thymidine kinase gene (tk) [Palella et al., Mol. Cell. Biol. 8 (1988) 457-460]. Infection of HPRT - cultured rat neuronal cells with these vectors resulted m transient expression of human hprt. In this paper, we report the expression of human hprt mRNA transcripts in the brains of mice infected in vivo with this vector by direct intracranial inoculation. Human hprt transcripts were distinguished from endogenous mouse transcripts by RNase A mapping using riboprobes transcribed from human hprt cDNA. These initial studies demonstrate the transfer and transcription of a human gene in brain cells by direct in vivo infection with recombinant HSV-1 vectors. | en_US |
dc.format.extent | 1077722 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Expression of human HPRT mRNA in brains of mice infected with a recombinant herpes simplex virus-1 vector | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A.; The Rackham Arthritis Research Unit, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | The Rackham Arthritis Research Unit, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A.; Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | The Rackham Arthritis Research Unit, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A.; Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A.; The Rackham Arthritis Research Unit, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A.; Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 2551779 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27810/1/0000215.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0378-1119(89)90258-8 | en_US |
dc.identifier.source | Gene | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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