Characterization of a topoisomerase-like activity at specific hypersensitive sites in the Drosophila histone gene cluster
dc.contributor.author | Villeponteau, Bryant | en_US |
dc.date.accessioned | 2006-04-07T20:45:30Z | |
dc.date.available | 2006-04-07T20:45:30Z | |
dc.date.issued | 1989-07-14 | en_US |
dc.identifier.citation | Villeponteau, Bryant (1989/07/14)."Characterization of a topoisomerase-like activity at specific hypersensitive sites in the Drosophila histone gene cluster." Biochemical and Biophysical Research Communications 162(1): 232-237. <http://hdl.handle.net/2027.42/27848> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4F031HH-25X/2/18b7f8848c01c29c8662493f9edca56f | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27848 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2546546&dopt=citation | en_US |
dc.description.abstract | It is well known that treatment of DNA-topoisomerase complexes with SDS induces cleavage of the DNA by trapping a reactive intermediate in which the topoisomerase is covalently linked to the terminal phosphates of the cut DNA. I have used this technique to examine potential topoisomerase binding sites in the histone gene chromatin of Drosophila Kc cells. Treatment of Kc nuclei with SDS induces Mg++-dependent DNA cleavage near the borders of two nuclease-hypersensitive sites located 5' and 3' of histone H4. It is likely that the SDS-induced cleavage at these hypersensitive sites is due to a topoisomerase because protein becomes tightly bound to the ends of the cleaved DNA fragments. Preliminary experiments suggest that a type II topoisomerase may be responsible for the cleavage. | en_US |
dc.format.extent | 1324646 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Characterization of a topoisomerase-like activity at specific hypersensitive sites in the Drosophila histone gene cluster | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The Institute of Gerontology, University of Michigan, 300 North Ingalls, Ann Arbor, MI 48109-2007, USA; Department of Biological Chemistry University of Michigan, 300 North Ingalls, Ann Arbor, MI 48109-2007, USA. | en_US |
dc.identifier.pmid | 2546546 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27848/1/0000259.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(89)91986-4 | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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