Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry
dc.contributor.author | Weng, Qing-Mei | en_US |
dc.contributor.author | Hammargren, William M. | en_US |
dc.contributor.author | Slowikowski, Debra | en_US |
dc.contributor.author | Schram, Karl H. | en_US |
dc.contributor.author | Borysko, Katherine Z. | en_US |
dc.contributor.author | Wotring, Linda L. | en_US |
dc.contributor.author | Townsend, Leroy B. | en_US |
dc.date.accessioned | 2006-04-07T20:50:44Z | |
dc.date.available | 2006-04-07T20:50:44Z | |
dc.date.issued | 1989-04 | en_US |
dc.identifier.citation | Weng, Qing-Mei, Hammargren, William M., Slowikowski, Debra, Schram, Karl H., Borysko, Katherine Z., Wotring, Linda L., Townsend, Leroy B. (1989/04)."Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry." Analytical Biochemistry 178(1): 102-106. <http://hdl.handle.net/2027.42/27979> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6W9V-4DYM9Y1-DG/2/8201cad7a9994f7d4df4aa1722725523 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/27979 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2729562&dopt=citation | en_US |
dc.description.abstract | The effect of trimethylsilyl (TMS) derivatization on detection limits of mononucleotides in fast atom bombardment-mass spectrometry (FAB-MS) was examined. FAB-MS methods were developed to optimize sensitivity using adenosine 5'-monophosphate as a model compound and then applied to reference standards of two clinically important nucleotides: tricyclic nucleoside-5'-monophosphate (TCNMP) and 5-fluoro-2'-deoxyuridine-5'-monophosphate (FdUMP). The detection limit for the TMS derivative of TCNMP was 2.5 - 5 ng/[mu]l and less than 2.5 ng/[mu]l for FdUMP as its TMS derivative. This is greater than two orders of magnitude more sensitive than the FAB-MS analysis of the corresponding free compounds. These low detection limits for the TMS derivatives were obtained using a narrow scan range, signal averaging, detection in the negative ion mode, and 3-nitrobenzyl alcohol as the matrix. Hydrolysis of one or more of the labile TMS groups did occur, with the extent of hydrolysis being greatest in the more protic matrices. | en_US |
dc.format.extent | 447057 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationum | College of Pharmacy, University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Department of Pharmaceutical Sciences, College of Pharmacy, University of Arizona, Tucson, Arizona 85721, USA | en_US |
dc.contributor.affiliationother | Department of Pharmaceutical Sciences, College of Pharmacy, University of Arizona, Tucson, Arizona 85721, USA | en_US |
dc.contributor.affiliationother | Department of Pharmaceutical Sciences, College of Pharmacy, University of Arizona, Tucson, Arizona 85721, USA | en_US |
dc.contributor.affiliationother | Department of Pharmaceutical Sciences, College of Pharmacy, University of Arizona, Tucson, Arizona 85721, USA | en_US |
dc.identifier.pmid | 2729562 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/27979/1/0000411.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-2697(89)90363-1 | en_US |
dc.identifier.source | Analytical Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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