Purification of the pancreatic cholecystokinin receptor
dc.contributor.author | Szecowka, J. | en_US |
dc.contributor.author | Hallden, G. | en_US |
dc.contributor.author | Goldfine, I. D. | en_US |
dc.contributor.author | Williams, John A. | en_US |
dc.date.accessioned | 2006-04-07T20:52:45Z | |
dc.date.available | 2006-04-07T20:52:45Z | |
dc.date.issued | 1989-03 | en_US |
dc.identifier.citation | Szecowka, J., Hallden, G., Goldfine, I. D., Williams, J. A. (1989/03)."Purification of the pancreatic cholecystokinin receptor." Regulatory Peptides 24(3): 215-224. <http://hdl.handle.net/2027.42/28032> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T0S-47V9HN1-X4/2/22c73dcbb5658a69ab555770192f016d | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28032 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2710962&dopt=citation | en_US |
dc.description.abstract | We have previously shown that the pancreatic cholecystokinin (CCK) receptor can be solubilized in 1% digitonin. In this study, digitonin-solubilized CCK receptors from rat pancreas were purified using sequential affinity chromatography on ricin-II agarose and on AffiGel-CCK. Electrophoresis of the radioiodinated purified receptors on SDS-polyacrylamide gels followed by autoradiography revealed two proteins: a major band of Mr = 80,000-90,000, and a minor band of Mr = 55,000. Through the purification procedure, the receptors preserved their agonist specificity (CCK-8 Kd = 9.4 nM. The estimated purification was about 80,000 fold and consistent with the expected Bmax for a pure Mr = 80,000 protein binding one CCK molecule. This two-step purification procedure opens the possibility for molecular studies of the CCK receptor. | en_US |
dc.format.extent | 615508 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Purification of the pancreatic cholecystokinin receptor | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Cell Biological Laboratory, Mount Zion Hospital and Medical Center, San Francisco, CA 94120, U.S.A.; Departments of Physiology and Medicine, University of California, San Francisco, CA 94143, U.S.A.; Departments of Physiology and Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Cell Biological Laboratory, Mount Zion Hospital and Medical Center, San Francisco, CA 94120, U.S.A. | en_US |
dc.contributor.affiliationother | Cell Biological Laboratory, Mount Zion Hospital and Medical Center, San Francisco, CA 94120, U.S.A. | en_US |
dc.contributor.affiliationother | Departments of Physiology and Medicine, University of California, San Francisco, CA 94143, U.S.A.; Cell Biological Laboratory, Mount Zion Hospital and Medical Center, San Francisco, CA 94120, U.S.A. | en_US |
dc.identifier.pmid | 2710962 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28032/1/0000471.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0167-0115(89)90218-8 | en_US |
dc.identifier.source | Regulatory Peptides | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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