Ontogeny of glutamate accumulating activity in rat brain synaptic vesicles
dc.contributor.author | Kish, Phillip E. | en_US |
dc.contributor.author | Kim, Soo Y. | en_US |
dc.contributor.author | Ueda, Tetsufumi | en_US |
dc.date.accessioned | 2006-04-07T20:53:36Z | |
dc.date.available | 2006-04-07T20:53:36Z | |
dc.date.issued | 1989-02-13 | en_US |
dc.identifier.citation | Kish, Phillip E., Kim, Soo Y., Ueda, Tetsufumi (1989/02/13)."Ontogeny of glutamate accumulating activity in rat brain synaptic vesicles." Neuroscience Letters 97(1-2): 185-190. <http://hdl.handle.net/2027.42/28055> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T0G-485YFS5-P0/2/327e7f0d6ec229744f08056b09ea1103 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28055 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2563905&dopt=citation | en_US |
dc.description.abstract | The relationship between the ontogeny of the vesicular glutamate uptake system and synaptogenesis in rats was investigated. For this purpose we have developed a simplified procedure for the preparation of crude synaptic vesicles which are sufficiently pure to demonstrate a highly ATP-dependent glutamate uptake. ATP-dependent glutamate uptake into synaptic vesicles was found to increase dramatically starting on postnatal day 10 and reaching a maximum on day 30 (76 +/- 40 and 657 +/- 40 pmol/mg protein/10 min, respectively), correlating well with the active period of synaptogenesis. Stimulation of uptake by chloride also developed in parallel with the vesicular glutamate uptake. In contrast, combined non-ATP-dependent uptake and non-specific binding remained constant (21 +/- 6 pmol/mg protein/10 min). This development of vesicular glutamate uptake during the period of synaptogenesis supports the notion that synaptic vesicles play an important role in glutamate synaptic transmission. | en_US |
dc.format.extent | 342942 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Ontogeny of glutamate accumulating activity in rat brain synaptic vesicles | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, The University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, The University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, The University of Michigan, Ann Arbor, MI 48109, U.S.A.; Department of Pharmacology, The University of Michigan, Ann Arbor, MI 48109, U.S.A.; Department of Psychiatry, The University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 2563905 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28055/1/0000494.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0304-3940(89)90161-4 | en_US |
dc.identifier.source | Neuroscience Letters | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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