Localization and characterization of somatostatin binding sites in the mouse retina
dc.contributor.author | Kossut, Malgorzata | en_US |
dc.contributor.author | Yamada, Tadataka | en_US |
dc.contributor.author | Aldrich, Leslie B. | en_US |
dc.contributor.author | Pinto, Lawrence H. | en_US |
dc.date.accessioned | 2006-04-07T20:55:06Z | |
dc.date.available | 2006-04-07T20:55:06Z | |
dc.date.issued | 1989-01-02 | en_US |
dc.identifier.citation | Kossut, Malgorzata, Yamada, Tadataka, Aldrich, Leslie B., Pinto, Lawrence H. (1989/01/02)."Localization and characterization of somatostatin binding sites in the mouse retina." Brain Research 476(1): 78-84. <http://hdl.handle.net/2027.42/28093> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6SYR-485PBTH-1B7/2/10317c69a661381c7447be5a7de8191b | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28093 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2563334&dopt=citation | en_US |
dc.description.abstract | We studied the binding of [125I]Tyr11-somatostatin-14 and [125I]Leu8,-Trp22, Tyr25-somatostatin-28 to frozen, unfixed sections of C57BL/6J mouse eyes with autoradiography. Specific binding of both ligands occurred in 3 maxima, a broad band extending from the retinal ganglion cell to the inner nuclear layers, a narrow and inconstant band over the outer plexiform layer, and a band over the retinal pigment epithelium and choroid. We quantified the label over the inner plexiform layer and found evidence for a single, saturable binding site after Scatchard analysis of saturation binding data. With [125I]Tyr11-somatostatin-14 the dissociation constant (Kd) was 1.48 nM and the total number of binding sites (Bmax) was 68 fmol/mg protein; in competition experiments the inhibitory binding constant (Ki) was 900 pM for somatostatin-14 and 350 pM for somatostatin-28. With [125I]Leu8,-Trp22, Tyr25-somatostatin-28, Kd was 625 pM and Bmax was 69 fmol/mg protein: in competition experiments Ki was 4.58 nM for somatostatin-14 and 710 pM for somatostatin-28. These results demonstrate the existence of somatostatin receptors in the inner plexiform layer of the retina that appear to have greater specificity for somatostatin-28 than for somatostatin-14. | en_US |
dc.format.extent | 589648 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Localization and characterization of somatostatin binding sites in the mouse retina | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, Gastroenterology Division, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, Gastroenterology Division, University of Michigan Medical Center, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, U.S.A. | en_US |
dc.identifier.pmid | 2563334 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28093/1/0000540.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-8993(89)91538-2 | en_US |
dc.identifier.source | Brain Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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