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Site-specific radiolabeling of monoclonal antibodies with biotin/streptavidin
dc.contributor.authorDel Rosario, Renato B.en_US
dc.contributor.authorWahl, Richard L.en_US
dc.date.accessioned2006-04-07T20:59:11Z
dc.date.available2006-04-07T20:59:11Z
dc.date.issued1989en_US
dc.identifier.citationDel Rosario, Renato B., Wahl, Richard L. (1989)."Site-specific radiolabeling of monoclonal antibodies with biotin/streptavidin." International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology 16(5): 525-527. <http://hdl.handle.net/2027.42/28202>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B7GH9-4C00NJG-6R/2/63256fef0d1f52ea5f100b2b2d269636en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/28202
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2807958&dopt=citationen_US
dc.description.abstractSulfhydryl groups of a murine IgG2a monoclonal antibody, 5G6.4, were site-specifically biotinylated with N-iodo-acetyl-N'-biotinylhexylenediamine (compound 1) following partial reduction of disulfide bonds. Antibody labeling using fluoresceinated or [125I]streptavidin were consistent with sulfhydryl group-specific incorporation of label with good retention of immunoreactivity, mainly in the form of high molecular weight oligomers (molecular weight [approximate] &gt; 400,000). In vivo localization of the radioantibody complex to xenografts of ovarian carcinoma was achieved. This approach appears to be a promising method of labeling monoclonal antibodies.en_US
dc.format.extent318997 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleSite-specific radiolabeling of monoclonal antibodies with biotin/streptavidinen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPhysicsen_US
dc.subject.hlbsecondlevelNuclear Engineering and Radiological Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDivision of Nuclear Medicine, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109-0028, U.S.A.en_US
dc.contributor.affiliationumDivision of Nuclear Medicine, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI 48109-0028, U.S.A.en_US
dc.identifier.pmid2807958en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/28202/1/0000655.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0883-2897(89)90067-6en_US
dc.identifier.sourceInternational Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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