Glucosylceramide synthase of mouse kidney: Further characterization with an improved assay method
dc.contributor.author | Shukla, Girja S. | en_US |
dc.contributor.author | Radin, Norman S. | en_US |
dc.date.accessioned | 2006-04-10T13:32:53Z | |
dc.date.available | 2006-04-10T13:32:53Z | |
dc.date.issued | 1990-12 | en_US |
dc.identifier.citation | Shukla, Girja S., Radin, Norman S. (1990/12)."Glucosylceramide synthase of mouse kidney: Further characterization with an improved assay method." Archives of Biochemistry and Biophysics 283(2): 372-378. <http://hdl.handle.net/2027.42/28291> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WB5-4DPC2PV-19D/2/39246db1a925a680b521dd210fb00608 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28291 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2148864&dopt=citation | en_US |
dc.description.abstract | The synthesis of glucosylceramide from ceramide and UDP-[3H]glucose by mouse kidney homogenates is very sensitive to the concentration of tissue. This was shown to be due to the presence of a UDP-glc pyrophosphatase, which could be blocked by adding NAD to the medium. A new solvent partitioning system is described, containing t-butyl methyl ether, isopropyl alcohol, and aqueous sodium sulfate, which separates the original substrate (UDP-[3H]glc) from the enzyme product, [3H]cerebroside. A particular advantage of the solvent system is that only a single partitioning step is needed, without backwashes, and the enzyme product appears in the upper phase, making transfer to a counting vial more reliable. A novel incubation device, a thermostatically controlled ultrasonic bath, is used to produce highly uniform enzyme reaction rates. Ca2+, as well as Mg2+ and Mn2+, was found to be a good stimulator of the glucosyltransferase. The enzyme activity in kidney of 22-day old mice, ~240 pmol/h/mg tissue, is significantly greater than previously demonstrated. The enzyme was stable in intact kidneys stored at -70 [deg]C but unstable at 4 [deg]C. The enzyme, when acting on endogenous ceramides, showed no demonstrable glucosylation of the C24 family of ceramides although this family is the predominant one in kidney. | en_US |
dc.format.extent | 773163 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Glucosylceramide synthase of mouse kidney: Further characterization with an improved assay method | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, The University of Michigan, Ann Arbor, Michigan 48104-1687, U.S.A. | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, The University of Michigan, Ann Arbor, Michigan 48104-1687, U.S.A. | en_US |
dc.identifier.pmid | 2148864 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28291/1/0000045.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0003-9861(90)90657-K | en_US |
dc.identifier.source | Archives of Biochemistry and Biophysics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.