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Retinoic acid and synthetic analogs differentially activate retinoic acid receptor dependent transcription
dc.contributor.authorAstrom, Andersen_US
dc.contributor.authorPettersson, Ulrikaen_US
dc.contributor.authorKrust, Andreeen_US
dc.contributor.authorChambon, Pierreen_US
dc.contributor.authorVoorhees, John J.en_US
dc.date.accessioned2006-04-10T13:33:25Z
dc.date.available2006-04-10T13:33:25Z
dc.date.issued1990-11-30en_US
dc.identifier.citationAstrom, Anders, Pettersson, Ulrika, Krust, Andree, Chambon, Pierre, Voorhees, John J. (1990/11/30)."Retinoic acid and synthetic analogs differentially activate retinoic acid receptor dependent transcription." Biochemical and Biophysical Research Communications 173(1): 339-345. <http://hdl.handle.net/2027.42/28304>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WBK-4FW0C1P-NS/2/f778d23b6a50174962ef835309bfe046en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/28304
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2175182&dopt=citationen_US
dc.description.abstractSummaryWe have developed an assay where the potency of retinoids in retinoic acid receptor (RAR) mediated transcriptional activation can be rapidly evaluated. In this assay hRAR-[alpha], hRAR-[beta] and hRAR-[gamma] were expressed in CV-1 cells together with a reporter gene containing a retinoic acid responsive element (TRE3-tk-CAT). Concentrations required to obtain half-maximum induction (ED50 of CAT-activity were determined for several retinoids, e.g., all-trans-retinoic acid (RA), 13-cis-retinoic acid (13-cis-RA), arotinoid acid (TTNPB) and m-carboxy-arotinoid acid (m-carboxy-TTNPB, an inactive arotinoid analog). The ED50 values for RA decreased in the order of RAR-[alpha] (24 nM) &gt; RAR-[beta] (4.0 nM) &gt; RAR-[gamma] (1.3 nM), while the ED50 values for TTNPB and 13-cis-RA decreased in the order of RAR-[alpha] (6.5 nM, 190 nM) &gt; RAR-[gamma] (2.3 nM, 140 nM) &gt; RAR-[beta] (0.6 nM, 43 nM), respectively. No significant inductions were obtained when cells were treated with m-carboxy-TTNPB, even at 10 [mu]M concentrations. The fold induction of CAT-activity for all compounds tested decreased in the order of RAR-[alpha] &gt; RAR-[beta] &gt; RAR-[gamma].en_US
dc.format.extent471083 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleRetinoic acid and synthetic analogs differentially activate retinoic acid receptor dependent transcriptionen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Dermatology, University of Michigan, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartment of Dermatology, University of Michigan, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartment of Dermatology, University of Michigan, Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationotherLaboratoire de Genetique Moleculaire des Eucaryotes du CNRS and U.184 INSERM, Faculte de Medecine, Strasbourg, Franceen_US
dc.contributor.affiliationotherLaboratoire de Genetique Moleculaire des Eucaryotes du CNRS and U.184 INSERM, Faculte de Medecine, Strasbourg, Franceen_US
dc.identifier.pmid2175182en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/28304/1/0000058.pdfen_US
dc.identifier.sourceBiochemical and Biophysical Research Communicationsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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