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Characterization and regulation of phosphatidylglycerolphosphate phosphatase in Saccharomyces cerevisiae

dc.contributor.authorKelly, Beth L.en_US
dc.contributor.authorGreenberg, Miriam L.en_US
dc.date.accessioned2006-04-10T13:36:37Z
dc.date.available2006-04-10T13:36:37Z
dc.date.issued1990-09-18en_US
dc.identifier.citationKelly, Beth L., Greenberg, Miriam L. (1990/09/18)."Characterization and regulation of phosphatidylglycerolphosphate phosphatase in Saccharomyces cerevisiae." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 1046(2): 144-150. <http://hdl.handle.net/2027.42/28384>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T1X-47G37S7-9W/2/e8263a93df30e87ecc4e8d1ced5f13acen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/28384
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2171664&dopt=citationen_US
dc.description.abstractPhosphatidylglycerophosphatase (EC 3.1.3.27) activity was characterized in mitochondrial extracts from Saccharomyces cerevisiae. The enzyme has a pH optimum of 5.5. Maximum activity occurs in the presence of Triton X-100 (5 mM) and cobalt or magnesium ions (5 mM). The apparent Km for PGP is 14.6 [mu]M. The temperature optimum is between 50[deg]C and 60[deg]C. The enzyme is labile above 50[deg]C. The presence of inositol in growth media results in a slight but reproducible increase in PGPase activity in mitochondrial extracts from glucose-grown cells but not glycerol-grown cells. The inositol effect is not seen in crude cell extracts. Carbon source does not affect PGPase activity in mitochondrial extracts or in crude cell extracts.en_US
dc.format.extent844426 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleCharacterization and regulation of phosphatidylglycerolphosphate phosphatase in Saccharomyces cerevisiaeen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMaterials Science and Engineeringen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry and Cellular and Molecular Biology Program, University of Michigan Medical School, Ann Arbor, MI, U.S.A.en_US
dc.contributor.affiliationumno department foundDepartment of Biological Chemistry and Cellular and Molecular Biology Program, University of Michigan Medical School, Ann Arbor, MI, U.S.A.en_US
dc.identifier.pmid2171664en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/28384/1/0000157.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0005-2760(90)90181-Ven_US
dc.identifier.sourceBiochimica et Biophysica Actaen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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