Characterization and regulation of phosphatidylglycerolphosphate phosphatase in Saccharomyces cerevisiae
dc.contributor.author | Kelly, Beth L. | en_US |
dc.contributor.author | Greenberg, Miriam L. | en_US |
dc.date.accessioned | 2006-04-10T13:36:37Z | |
dc.date.available | 2006-04-10T13:36:37Z | |
dc.date.issued | 1990-09-18 | en_US |
dc.identifier.citation | Kelly, Beth L., Greenberg, Miriam L. (1990/09/18)."Characterization and regulation of phosphatidylglycerolphosphate phosphatase in Saccharomyces cerevisiae." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 1046(2): 144-150. <http://hdl.handle.net/2027.42/28384> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1X-47G37S7-9W/2/e8263a93df30e87ecc4e8d1ced5f13ac | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28384 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2171664&dopt=citation | en_US |
dc.description.abstract | Phosphatidylglycerophosphatase (EC 3.1.3.27) activity was characterized in mitochondrial extracts from Saccharomyces cerevisiae. The enzyme has a pH optimum of 5.5. Maximum activity occurs in the presence of Triton X-100 (5 mM) and cobalt or magnesium ions (5 mM). The apparent Km for PGP is 14.6 [mu]M. The temperature optimum is between 50[deg]C and 60[deg]C. The enzyme is labile above 50[deg]C. The presence of inositol in growth media results in a slight but reproducible increase in PGPase activity in mitochondrial extracts from glucose-grown cells but not glycerol-grown cells. The inositol effect is not seen in crude cell extracts. Carbon source does not affect PGPase activity in mitochondrial extracts or in crude cell extracts. | en_US |
dc.format.extent | 844426 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Characterization and regulation of phosphatidylglycerolphosphate phosphatase in Saccharomyces cerevisiae | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry and Cellular and Molecular Biology Program, University of Michigan Medical School, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | no department foundDepartment of Biological Chemistry and Cellular and Molecular Biology Program, University of Michigan Medical School, Ann Arbor, MI, U.S.A. | en_US |
dc.identifier.pmid | 2171664 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28384/1/0000157.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0005-2760(90)90181-V | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.