Acetylcholine, carbachol, and GABA induce no detectable change in the length of isolated outer hair cells
dc.contributor.author | Bobbin, Richard P. | en_US |
dc.contributor.author | Fallon, Maureen | en_US |
dc.contributor.author | Puel, Jean-Luc | en_US |
dc.contributor.author | Bryant, Georgia | en_US |
dc.contributor.author | Bledsoe, Sanford C., Jr. | en_US |
dc.contributor.author | Zajic, Gary | en_US |
dc.contributor.author | Schacht, Jochen | en_US |
dc.date.accessioned | 2006-04-10T13:38:32Z | |
dc.date.available | 2006-04-10T13:38:32Z | |
dc.date.issued | 1990-08-01 | en_US |
dc.identifier.citation | Bobbin, Richard P., Fallon, Maureen, Puel, Jean-Luc, Bryant, Georgia, Bledsoe, Jr., Sanford C., Zajic, Gary, Schacht, Jochen (1990/08/01)."Acetylcholine, carbachol, and GABA induce no detectable change in the length of isolated outer hair cells." Hearing Research 47(1-2): 39-52. <http://hdl.handle.net/2027.42/28432> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T73-4864N9H-CJ/2/109c3c33526f1e61b96f89895371711b | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28432 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2228797&dopt=citation | en_US |
dc.description.abstract | The mechanical and electrical properties of cochlear outer hair cells (OHCs) are suggested to modulate transduction by inner hair cells. These properties of OHCs are presumably regulated by efferent neurons which use several transmitters including acetylcholine (Ach) and gamma aminobutyric acid (GABA). Since it had been suggested that Ach causes isolated OHCs to shorten visibly, this study was designed to investigate whether GABA also alters the length of OHCs. OHCs were isolated from the guinea pig cochlea by mechanical dispersion after collagenase treatment. Cells were initially selected by strict morphological criteria. In addition they were only included in further studies if they attained a constant length during 10 min of superfusion with buffer solution. Neither GABA (20 [mu]M; 100 [mu]M), Ach (5 mM; 10 [mu]M with 10 [mu]M eserine) or carbachol (10 [mu]M; 100 [mu]M) altered OHC length when applied in iso-osmotic Hank's balanced salt solution (total number of cells tested, 72). If a change in length occurred it must have been smaller than 0.3 [mu]m, our detection ability. In contrast, high potassium and variations in osmolarity changed hair cell length by 3-10% in agreement with other reports. | en_US |
dc.format.extent | 1329527 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Acetylcholine, carbachol, and GABA induce no detectable change in the length of isolated outer hair cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, The University of Michigan, Department of Otolaryngology, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, The University of Michigan, Department of Otolaryngology, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, The University of Michigan, Department of Otolaryngology, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationother | Louisiana State University Medical School, Kresge Hearing Research Laboratory of the South, Department of Otolaryngology and Biocommunication, New Orleans, Lousiana, U.S.A. | en_US |
dc.contributor.affiliationother | Louisiana State University Medical School, Kresge Hearing Research Laboratory of the South, Department of Otolaryngology and Biocommunication, New Orleans, Lousiana, U.S.A. | en_US |
dc.contributor.affiliationother | Louisiana State University Medical School, Kresge Hearing Research Laboratory of the South, Department of Otolaryngology and Biocommunication, New Orleans, Lousiana, U.S.A. | en_US |
dc.contributor.affiliationother | Louisiana State University Medical School, Kresge Hearing Research Laboratory of the South, Department of Otolaryngology and Biocommunication, New Orleans, Lousiana, U.S.A. | en_US |
dc.identifier.pmid | 2228797 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28432/1/0000219.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0378-5955(90)90165-L | en_US |
dc.identifier.source | Hearing Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.