Insulin stimulates protein synthesis and phospholipid signaling systems but does not regulate glucose uptake in the inner ear
dc.contributor.author | Wang, Shuchun | en_US |
dc.contributor.author | Schacht, Jochen | en_US |
dc.date.accessioned | 2006-04-10T13:38:34Z | |
dc.date.available | 2006-04-10T13:38:34Z | |
dc.date.issued | 1990-08-01 | en_US |
dc.identifier.citation | Wang, Shuchun, Schacht, Jochen (1990/08/01)."Insulin stimulates protein synthesis and phospholipid signaling systems but does not regulate glucose uptake in the inner ear." Hearing Research 47(1-2): 53-61. <http://hdl.handle.net/2027.42/28433> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T73-4864N9H-CK/2/fcb92926a60ad6c2b2bfbf73bd6fda02 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28433 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2228798&dopt=citation | en_US |
dc.description.abstract | High-affinity insulin receptors exist in the organ of Corti (Kd = 1.1 +/-0.5 nM) and in the lateral wall (stria vascularis and ligamentum spirale; Kd = 1.1 +/-0.4 nM) of the inner ear of the guinea pig as determined by the binding of radiolabeled porcine or bovine insulin in vitro. Carrier-mediated transport of glucose (defined as the cytochalasin B-sensitive part of total uptake) was measured in vitro with 2-deoxy--glucose as the substrate. Its Km was 188 [mu]M in the organ of Corti, and 41 [mu]M in the lateral wall (r = 0.99 and 0.94, respectively). Neither the Km nor the rates of transport (0.20 +/- 0.10 pmol/[mu]g protein/hr in the organ of Co and 0.56 +/- 0.34 pmol/[mu]g protein/hr in the lateral wall) were affected by insulin. In contrast, 0.1 mM ouabain decreased deoxyglucose uptake in the organ of Corti by 37% and in the lateral wall tissues by 45% indicating the presence of an active, Na+-dependent transporter in these tissues.Insulin influenced both protein and lipid metabolism in the inner ear. Proteins and lipids were labeled in situ by perfusion of the perilymphatic space of the cochlea with [3H]-leucine or [32P]-orthophosphate and [3H]-glycerol, respectively. Thirty nM insulin stimulated the incorporation of [3H]-leucine into protein of the organ of Corti from 39 to 56 pmol /mg protein but was ineffective in the tissues of the lateral wall. In the organ of Corti, [32P]-orthophosphate was incorporated into the phosphoinositides and phosphatidate, and 30 nM insulin increased this incorporation by 101 to 149%. With [3H]-glycerol, a trend towards increased incorporation in the presence of insulin was also evident for neutral lipids, phosphatidate, and phosphatidylinositol. In contrast, labeling of phosphatidylcholine (including phosphatidylethanolamine) was reduced. The lateral wall tissues showed a similar pattern of incorporation and a lesser stimulation by insulin. The evidence presented is consistent with a phospholipid-based transmembrane signaling system mediating the effects of insulin on inner ear tissues. | en_US |
dc.format.extent | 858598 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Insulin stimulates protein synthesis and phospholipid signaling systems but does not regulate glucose uptake in the inner ear | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, The University of Michigan, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 2228798 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28433/1/0000220.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0378-5955(90)90166-M | en_US |
dc.identifier.source | Hearing Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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