Comparison of assays for gap junctional communication using human embryocarcinoma cells exposed to dieldrin
dc.contributor.author | Loch-Caruso, Rita | en_US |
dc.contributor.author | Caldwell, V. | en_US |
dc.contributor.author | Cimini, M. | en_US |
dc.contributor.author | Juberg, Daland R. | en_US |
dc.date.accessioned | 2006-04-10T13:40:33Z | |
dc.date.available | 2006-04-10T13:40:33Z | |
dc.date.issued | 1990-07 | en_US |
dc.identifier.citation | Loch-Caruso, R., Caldwell, V., Cimini, M., Juberg, D. (1990/07)."Comparison of assays for gap junctional communication using human embryocarcinoma cells exposed to dieldrin." Fundamental and Applied Toxicology 15(1): 63-74. <http://hdl.handle.net/2027.42/28483> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WFT-4DDP5P3-B8/2/feb23a4bd3c8610704f3677070773695 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28483 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2197146&dopt=citation | en_US |
dc.description.abstract | Several assays were compared for their ability to detect inhibition of gap junctional communication in human embryocarcinoma cells exposed to the pesticide dieldrin. Included in this evaluation was a recently developed assay based on the transfer of the fluorescent dye derived metabolically from 5(and 6)-carboxy-2,7-dichlorofluorescein diacetate. This assay was compared to assays for fluorescence return after photobleaching (FRAP), transfer of Lucifer yellow after scrape-loading, autoradiographic visualization of [3H]-uridine nucleotide transfer, and metabolic coupling of 6-thioguanine (6-TG) metabolites. The scrape-loading assay was the most sensitive assay, detecting inhibition of junctional communication at all concentrations tested. Additionally, the scrape-loading assay provided the clearest demonstration of concentration-dependent inhibition of junctional communication, although the [3H]uridine assay also showed significant concentration-related effects. The 5(and 6)-carboxy-2,7-dichlorofluorescein diacetate and 6-TG metabolic coupling assays detected significant inhibition at the two highest concentrations of dieldrin only. The FRAP assay also detected substantial inhibition at the two highest concentrations only. These results show that scrapeloading is the most sensitive assay of those compared in this study for the detection of inhibited junctional communication. Furthermore, compared to the other assays evaluated, the newly developed 5(and 6)-carboxy-2,7-dichlorofluorescein diacetate assay is at least as sensitive, yet less cumbersome, less expensive, and more rapid. Finally, the results show that each of these assays was easily applied to embryonic cells, suggesting that they may be useful for evaluating disruption of junctional communication in embryonic cell cultures. | en_US |
dc.format.extent | 4681968 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Comparison of assays for gap junctional communication using human embryocarcinoma cells exposed to dieldrin | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Pharmacy and Pharmacology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Toxicology Program, Department of Environmental and Industrial Health, The University of Michigan, Ann Arbor, Michigan 48109-2029, USA | en_US |
dc.contributor.affiliationum | Toxicology Program, Department of Environmental and Industrial Health, The University of Michigan, Ann Arbor, Michigan 48109-2029, USA | en_US |
dc.contributor.affiliationum | Toxicology Program, Department of Environmental and Industrial Health, The University of Michigan, Ann Arbor, Michigan 48109-2029, USA | en_US |
dc.contributor.affiliationum | Toxicology Program, Department of Environmental and Industrial Health, The University of Michigan, Ann Arbor, Michigan 48109-2029, USA | en_US |
dc.identifier.pmid | 2197146 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28483/1/0000277.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0272-0590(90)90163-E | en_US |
dc.identifier.source | Fundamental and Applied Toxicology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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