A simple dye-coupling assay for evaluating gap junctional communication: The importance of transcription and translation on the establishment of dye-coupling
dc.contributor.author | Zidell, Robert H. | en_US |
dc.contributor.author | Loch-Caruso, Rita | en_US |
dc.date.accessioned | 2006-04-10T13:41:45Z | |
dc.date.available | 2006-04-10T13:41:45Z | |
dc.date.issued | 1990-07 | en_US |
dc.identifier.citation | Zidell, Robert H., Loch-Caruso, Rita (1990/07)."A simple dye-coupling assay for evaluating gap junctional communication: The importance of transcription and translation on the establishment of dye-coupling." Cell Biology International Reports 14(7): 613-628. <http://hdl.handle.net/2027.42/28513> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B7MF6-4DXRY6V-6T/2/67cb53ebd75fb58667b38fd386018026 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28513 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2393907&dopt=citation | en_US |
dc.description.abstract | A simple dye-coupling assay has been developed to study gap junctional communication using a uterine cell line. The new dye-coupling assay was found to be of comparable sensitivity to an autoradiographic assay for transfer of radiolabelled nucleotide. To investigate the importance of protein synthesis in the establishment of dye-coupling under the conditions of this assay, cultures were treated with actinomycin-D and cycloheximide to inhibit transcription and translation, respectively. Inhibition of transcription had no significant effect on dye-coupling. The protein synthesis inhibitor, cycloheximide, also had no significant effect on dye-coupling at concentrations up to 1 uM, even though protein synthesis was inhibited to 19% of control. | en_US |
dc.format.extent | 1562766 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A simple dye-coupling assay for evaluating gap junctional communication: The importance of transcription and translation on the establishment of dye-coupling | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Toxicology Program, Department of Environmental and Industrial Health, The University of Michigan School of Public Health, 109 S. Observatory, Ann Arbor, MI 48109-2029, U.S.A. | en_US |
dc.contributor.affiliationum | Toxicology Program, Department of Environmental and Industrial Health, The University of Michigan School of Public Health, 109 S. Observatory, Ann Arbor, MI 48109-2029, U.S.A. | en_US |
dc.identifier.pmid | 2393907 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28513/1/0000310.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0309-1651(90)90041-V | en_US |
dc.identifier.source | Cell Biology International Reports | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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