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Cytosolic free calcium spiking affected by intracellular pH change

dc.contributor.authorTsunoda, Yasuhiroen_US
dc.date.accessioned2006-04-10T13:43:01Z
dc.date.available2006-04-10T13:43:01Z
dc.date.issued1990-06en_US
dc.identifier.citationTsunoda, Yasuhiro (1990/06)."Cytosolic free calcium spiking affected by intracellular pH change." Experimental Cell Research 188(2): 294-301. <http://hdl.handle.net/2027.42/28545>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WFC-4DVV4MF-JS/2/1402490c1946f1c40e71a753fb14d2c1en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/28545
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2335190&dopt=citationen_US
dc.description.abstractThe characteristics underlying cytosolic free calcium oscillation were evaluated by superfused dual wavelength microspectrofluorometry of fura-2-loaded single acinar cells from rat pancreas. Application of a physiological concentration of cholecystokinin octapeptide (CCK) (20 pM) induced a small basal increase in cytosolic free calcium concentration ([Ca2+]i) averaging 34 nM above the prestimulation level (69 nM) with superimposed repetitive Ca2+ spike oscillation. The oscillation amplitude averaged 121 nM above the basal increase in [Ca2+]i and occurred at a frequency of one pulse every 49 s. Although extracellular Ca2+ was required for maintenance of high frequency and amplitude of the spikes with increase in basal [Ca2+]i, the primary source utilized for oscillation was intracellular. The threshold of the peak [Ca2+]i amplitude for causing synchronized and same-sized oscillations was less than 300 nM. The [Ca2+]i oscillation was sensitive to intracellular pH (pHi) change. This is shown by the fact that the large pHi shift toward acidification ([Delta]pHi decrease, 0.95) led to a basal increase in [Ca2+]i to the spike peak level with inhibiting Ca2+ oscillation. The pHi shift toward alkalinization ([Delta]pHi increase, 0.33) led to a basal decrease in [Ca2+]i to the prestimulation level, possibly due to reuptake of Ca2+ into the Ca2+ stores, with inhibiting Ca2+ oscillation. Whereas extracellular pH (pHo) change had only minimal effects on Ca2+ oscillation (and/or Ca2+ release from intracellular stores), the extra-Ca2+ entry process, which was induced by higher concentrations of CCK, was totally inhibited by decreasing pHo from 7.4 to 6.5. Thus the major regulatory sites by which H+ affects Ca2+ oscillation are accessible from the intracellular space.en_US
dc.format.extent887630 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleCytosolic free calcium spiking affected by intracellular pH changeen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Physiology, Med. Sci. II, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid2335190en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/28545/1/0000344.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0014-4827(90)90173-8en_US
dc.identifier.sourceExperimental Cell Researchen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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