Cytosolic free calcium spiking affected by intracellular pH change
dc.contributor.author | Tsunoda, Yasuhiro | en_US |
dc.date.accessioned | 2006-04-10T13:43:01Z | |
dc.date.available | 2006-04-10T13:43:01Z | |
dc.date.issued | 1990-06 | en_US |
dc.identifier.citation | Tsunoda, Yasuhiro (1990/06)."Cytosolic free calcium spiking affected by intracellular pH change." Experimental Cell Research 188(2): 294-301. <http://hdl.handle.net/2027.42/28545> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WFC-4DVV4MF-JS/2/1402490c1946f1c40e71a753fb14d2c1 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28545 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2335190&dopt=citation | en_US |
dc.description.abstract | The characteristics underlying cytosolic free calcium oscillation were evaluated by superfused dual wavelength microspectrofluorometry of fura-2-loaded single acinar cells from rat pancreas. Application of a physiological concentration of cholecystokinin octapeptide (CCK) (20 pM) induced a small basal increase in cytosolic free calcium concentration ([Ca2+]i) averaging 34 nM above the prestimulation level (69 nM) with superimposed repetitive Ca2+ spike oscillation. The oscillation amplitude averaged 121 nM above the basal increase in [Ca2+]i and occurred at a frequency of one pulse every 49 s. Although extracellular Ca2+ was required for maintenance of high frequency and amplitude of the spikes with increase in basal [Ca2+]i, the primary source utilized for oscillation was intracellular. The threshold of the peak [Ca2+]i amplitude for causing synchronized and same-sized oscillations was less than 300 nM. The [Ca2+]i oscillation was sensitive to intracellular pH (pHi) change. This is shown by the fact that the large pHi shift toward acidification ([Delta]pHi decrease, 0.95) led to a basal increase in [Ca2+]i to the spike peak level with inhibiting Ca2+ oscillation. The pHi shift toward alkalinization ([Delta]pHi increase, 0.33) led to a basal decrease in [Ca2+]i to the prestimulation level, possibly due to reuptake of Ca2+ into the Ca2+ stores, with inhibiting Ca2+ oscillation. Whereas extracellular pH (pHo) change had only minimal effects on Ca2+ oscillation (and/or Ca2+ release from intracellular stores), the extra-Ca2+ entry process, which was induced by higher concentrations of CCK, was totally inhibited by decreasing pHo from 7.4 to 6.5. Thus the major regulatory sites by which H+ affects Ca2+ oscillation are accessible from the intracellular space. | en_US |
dc.format.extent | 887630 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Cytosolic free calcium spiking affected by intracellular pH change | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Physiology, Med. Sci. II, University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.identifier.pmid | 2335190 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28545/1/0000344.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0014-4827(90)90173-8 | en_US |
dc.identifier.source | Experimental Cell Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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