Isolation and sequence analysis of CDC43, a gene involved in the controol of cell polarity in Saccharomyces cerevisiae
dc.contributor.author | Johnson, Douglas I. | en_US |
dc.contributor.author | O'Brien, Jeanne M. | en_US |
dc.contributor.author | Jacobs, Charles W. | en_US |
dc.date.accessioned | 2006-04-10T13:43:33Z | |
dc.date.available | 2006-04-10T13:43:33Z | |
dc.date.issued | 1990-05-31 | en_US |
dc.identifier.citation | Johnson, Douglas I., O'Brien, Jeanne M., Jacobs, Charles W. (1990/05/31)."Isolation and sequence analysis of CDC43, a gene involved in the controol of cell polarity in Saccharomyces cerevisiae." Gene 90(1): 93-98. <http://hdl.handle.net/2027.42/28558> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T39-47GH971-F/2/9a6c4ed0e8866a9936aa51e6eefc3b1e | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28558 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2199333&dopt=citation | en_US |
dc.description.abstract | The Saccharomyces cerevisiae CDC43 gene product is involved in establishing cell polarity during the cell-division cycle. When grown at restrictive temperatures, temperature-sensitive cdc43 mutants are unable to form buds and display delocalized cell-surface deposition [Adams et al., J. Cell Biol. (1990) in press]. We have isolated a cdc43-complementing plasmid from a yeast genomic-DNA library and localized the CDC43 gene, by subcloning and transposon-mutagenesis experiments, to a 1.2-kb region of DNA that contained only one significant ATG-initiated open reading frame of 213 codons. The putative CDC43 gene product contains a possible nuclear-localization signal sequence a cysteine-rich domain and a histidine-rich domain, and a region that is similar in structure to [alpha]-helix-turn-[alpha]-helix structural domains present in some prokaryotic and eukaryotic DNA-binding proteins. | en_US |
dc.format.extent | 586726 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Isolation and sequence analysis of CDC43, a gene involved in the controol of cell polarity in Saccharomyces cerevisiae | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, U.S.A.; Department of Biology, The University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biology, The University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, U.S.A. | en_US |
dc.identifier.pmid | 2199333 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28558/1/0000360.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0378-1119(90)90443-U | en_US |
dc.identifier.source | Gene | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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