Protein separation using membrane-encapsulated soluble ligand conjugates
dc.contributor.author | Sakoda, Akiyoshi | en_US |
dc.contributor.author | Nigam, Somesh C. | en_US |
dc.contributor.author | Wang, Henry Y. | en_US |
dc.date.accessioned | 2006-04-10T13:45:22Z | |
dc.date.available | 2006-04-10T13:45:22Z | |
dc.date.issued | 1990-05 | en_US |
dc.identifier.citation | Sakoda, Akiyoshi, Nigam, Somesh C., Wang, Henry Y. (1990/05)."Protein separation using membrane-encapsulated soluble ligand conjugates." Enzyme and Microbial Technology 12(5): 349-354. <http://hdl.handle.net/2027.42/28604> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TG1-47DM54B-TJ/2/43407085e1d5bf4f2cc64bb028f6cd65 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28604 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1366487&dopt=citation | en_US |
dc.description.abstract | A new approach for isolating and recovering biological macromolecules using membrane-encapsulated soluble ligand conjugates was investigated. Membrane-encapsulated solid adsorbents have been successfully developed and employed in our laboratory to isolate and purify proteins and enzymes directly from culture broths. This new concept also makes it possible to use soluble ligand conjugates instead of solid adsorbents inside membrane capsules. In this work, model membrane-encapsulated soluble and insoluble ligands comprising Blue Dextran and Blue Sepharose entrapped within calcium alginate membranes were studied to compare adsorption characteristics such as capacities and rates. Experimental results suggest that membrane-encapsulated soluble ligands may be expected to result in higher overall adsorption capacity compared to membrane-encapsulated solid adsorbents with comparable adsorption rates. | en_US |
dc.format.extent | 725273 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Protein separation using membrane-encapsulated soluble ligand conjugates | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.identifier.pmid | 1366487 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28604/1/0000413.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0141-0229(90)90163-K | en_US |
dc.identifier.source | Enzyme and Microbial Technology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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