Isolation of a catalytically competent phosphorylated tyrosine kinase from Rous sarcoma virus-induced rat tumor by immunoadsorption to and hapten elution from phosphotyrosine binding antibodies
dc.contributor.author | Whitehouse-Hills, Susan | en_US |
dc.contributor.author | Argetsinger, Lawrence S. | en_US |
dc.contributor.author | Shafer, Jules A. | en_US |
dc.date.accessioned | 2006-04-10T13:49:47Z | |
dc.date.available | 2006-04-10T13:49:47Z | |
dc.date.issued | 1990-03-01 | en_US |
dc.identifier.citation | Whitehouse-Hills, Susan, Argetsinger, Lawrence S., Shafer, Jules A. (1990/03/01)."Isolation of a catalytically competent phosphorylated tyrosine kinase from Rous sarcoma virus-induced rat tumor by immunoadsorption to and hapten elution from phosphotyrosine binding antibodies." Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1037(3): 380-387. <http://hdl.handle.net/2027.42/28715> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T21-47PNWY2-4C/2/4858b61f7a4dad130d272c6b11a02055 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28715 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1690027&dopt=citation | en_US |
dc.description.abstract | A procedure has been developed for the isolation of a catalytically competent phosphorylated tyrosine kinase (RSV Y-kinase) from avian sarcoma virus-induced rat tumors. The procedure involves reaction of partially purified RSV Y-kinase with ATP to effect tyrosyl phosphorylation of catalytically competent RSV Y-kinase. Tyrosyl phosphorylated RSV Y-kinase was isolated from the heterogenous reaction mixture by immunoadsorption on immobilized phosphotyrosyl binding antibodies and elution with the hapten p-nitrophenyl phosphate. Estimation of the phosphate content of the purified phosphorylated RSV Y-kinase indicated that 1-3 tyrosyl groups had been phosphorylated upon reaction with ATP. The specific activity toward histone 2B of the purified phosphorylated RSV Y-kinase was at least 30-fold greater than that estimated for the RSV Y-kinase prepared previously by immunoadsorption on immobilized antiserum from tumor bearing rabbits. | en_US |
dc.format.extent | 1140304 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Isolation of a catalytically competent phosphorylated tyrosine kinase from Rous sarcoma virus-induced rat tumor by immunoadsorption to and hapten elution from phosphotyrosine binding antibodies | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, The University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationother | Brain Tumor Research Center, The University of California, San Francisco, CA, U.S.A. | en_US |
dc.identifier.pmid | 1690027 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28715/1/0000536.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0167-4838(90)90040-M | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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