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Crystallization and preliminary X-ray characterization of a soybean seed lipoxygenase
dc.contributor.authorStallings, William C.en_US
dc.contributor.authorKroa, Beth A.en_US
dc.contributor.authorCarroll, Richard T.en_US
dc.contributor.authorMetzger, Anita L.en_US
dc.contributor.authorFunk, Max O.en_US
dc.date.accessioned2006-04-10T13:49:59Z
dc.date.available2006-04-10T13:49:59Z
dc.date.issued1990-02-20en_US
dc.identifier.citationStallings, William C., Kroa, Beth A., Carroll, Richard T., Metzger, Anita L., Funk, Max O. (1990/02/20)."Crystallization and preliminary X-ray characterization of a soybean seed lipoxygenase." Journal of Molecular Biology 211(4): 685-687. <http://hdl.handle.net/2027.42/28720>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6WK7-4FNGGYM-5/2/99805b1020e748250cc8afb8c73d4b13en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/28720
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2313694&dopt=citationen_US
dc.description.abstractAn isoenzyme of soybean (Glycine max L. Merrill cv. Provar) lipoxygenase (EC 1.13.11.12) has been crystallized using the vapor diffusion method. Crystals were grown from solutions of the protein (7 mg/ml) using 10 to 20% (w/v) polyethylene glycol 8000 in citrate/phosphate buffer (pH 5.7) containing 0.5% (w/v) n-octyl-[beta]--glucopyranoside. The crystals reached maximum dimensions of 0.3 mm x 0.2 mm x &gt; 2mm. The enzyme crystallized in space group C2221 with unit cell dimensions a = 246 A, B = 193 A and C = 75 A. A calculated Vm value of 2.35 A3/dalton was obtained assuming two molecules per asymmetric unit. The density of the crystals was found to be 1.16 g/ml, which confirmed the presence of two molecules per asymmetric unit and indicated a solvent content of 47.5%.en_US
dc.format.extent316256 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleCrystallization and preliminary X-ray characterization of a soybean seed lipoxygenaseen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumBiophysics Research Division University of Michigan, Ann Arbor, MI 48109, U.S.A.en_US
dc.contributor.affiliationotherPhysical Sciences Center Monsanto Company, St Louis, MO 63198, U.S.A.en_US
dc.contributor.affiliationotherDepartment of Chemistry University of Toledo, Toledo, OH 43606, U.S.A.en_US
dc.contributor.affiliationotherDepartment of Chemistry University of Toledo, Toledo, OH 43606, U.S.A.en_US
dc.contributor.affiliationotherDepartment of Chemistry University of Toledo, Toledo, OH 43606, U.S.A.en_US
dc.identifier.pmid2313694en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/28720/1/0000541.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0022-2836(90)90067-Ven_US
dc.identifier.sourceJournal of Molecular Biologyen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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