Charge-coupled device imaging of rapid calcium transients in cultured arterial smooth muscle cells
dc.contributor.author | Linderman, Jennifer J. | en_US |
dc.contributor.author | Harris, L. J. | en_US |
dc.contributor.author | Slakey, L. L. | en_US |
dc.contributor.author | Gross, D. J. | en_US |
dc.date.accessioned | 2006-04-10T13:50:30Z | |
dc.date.available | 2006-04-10T13:50:30Z | |
dc.date.issued | 1990 | en_US |
dc.identifier.citation | Linderman, J. J., Harris, L. J., Slakey, L. L., Gross, D. J. (1990)."Charge-coupled device imaging of rapid calcium transients in cultured arterial smooth muscle cells." Cell Calcium 11(2-3): 131-144. <http://hdl.handle.net/2027.42/28733> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WCC-4C0CWJB-NW/2/988a0c0913776eb494af73831bb6b548 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28733 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2354497&dopt=citation | en_US |
dc.description.abstract | Transient changes in the concentration of intracellular free calcium are associated with the transduction of primary signals and the subsequent employment of Ca2+ as a second messenger in a multitude of cell types. These transients, typically monitored with the calcium-sensitive fluorescent dye Fura-2, are known to occur with a time course in the order of seconds. In order to accurately monitor such rapid changes in intracellular free calcium concentration in both single cells and simultaneously in several cells in a single field, we have developed a digital fluorescence imaging system based on a charge-coupled device (CCD) camera. We report here on the detailed kinetics of calcium increases in cultured arterial swine smooth muscle cells in response to the agonist ATP. | en_US |
dc.format.extent | 1536066 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Charge-coupled device imaging of rapid calcium transients in cultured arterial smooth muscle cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Dentistry | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biochemistry, University of Massachusetts, Amherst, Massachusetts, USA; Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, Massachusetts, USA; Department of Chemical Engineering, University of Michigan Ann Arbor, Michigan, USA. | en_US |
dc.contributor.affiliationother | Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, Massachusetts, USA; Department of Biochemistry, University of Massachusetts, Amherst, Massachusetts, USA. | en_US |
dc.contributor.affiliationother | Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, Massachusetts, USA; Department of Biochemistry, University of Massachusetts, Amherst, Massachusetts, USA. | en_US |
dc.contributor.affiliationother | Department of Biochemistry, University of Massachusetts, Amherst, Massachusetts, USA; Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, Massachusetts, USA. | en_US |
dc.identifier.pmid | 2354497 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28733/1/0000560.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0143-4160(90)90066-4 | en_US |
dc.identifier.source | Cell Calcium | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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