Rabbit nasal cytochrome P-450 NMa has high activity as a nicotine oxidase
dc.contributor.author | Williams, David E. | en_US |
dc.contributor.author | Ding, Xinxin | en_US |
dc.contributor.author | Coon, Minor J. | en_US |
dc.date.accessioned | 2006-04-10T13:51:03Z | |
dc.date.available | 2006-04-10T13:51:03Z | |
dc.date.issued | 1990-01-30 | en_US |
dc.identifier.citation | Williams, David E., Ding, Xinxin, Coon, Minor J. (1990/01/30)."Rabbit nasal cytochrome P-450 NMa has high activity as a nicotine oxidase." Biochemical and Biophysical Research Communications 166(2): 945-952. <http://hdl.handle.net/2027.42/28747> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4DMX9RM-155/2/738dbf11808dfc7ab5523d063669bd84 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28747 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2302249&dopt=citation | en_US |
dc.description.abstract | Rabbit nasal olfactory and respiratory microsomes demonstrate high activity toward [3H]-(S)-nicotine, with specific activities of 22.2 and 6.5 nmol/min/mg protein, respectively. The major metabolite produced is (S)-nicotine [Delta]I',5'-iminium ion, with lesser amounts of nornicotine and the N'-oxide. Reconstitution of the rabbit nasal microsomal system with cytochromes P-450 NMa and NMb indicated that only P-450 NMa has significant activity toward nicotine, and the metabolite profile and turnover are similar to that observed with nasal microsomes. The low Km (35 [mu]M) and high Vmax (28 min-1) suggest that a significant portion of inhaled nicotine is metabolized by nasal tissues in the rabbit. | en_US |
dc.format.extent | 575172 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Rabbit nasal cytochrome P-450 NMa has high activity as a nicotine oxidase | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, Medical School, The University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, Medical School, The University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationother | Department of Food Science & Technology, Oregon State University, Corvallis, OR 97331, USA; Toxicology Program, Oregon State University, Corvallis, OR 97331, USA. | en_US |
dc.identifier.pmid | 2302249 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28747/1/0000577.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(90)90902-Y | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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