Rapid flow-injection sandwich-type immunoassays of proteins using an immobilized antibody reactor and adenosine deaminase-antibody conjugates
dc.contributor.author | Lee, Ing Huei | en_US |
dc.contributor.author | Meyerhoff, Mark E. | en_US |
dc.date.accessioned | 2006-04-10T13:52:29Z | |
dc.date.available | 2006-04-10T13:52:29Z | |
dc.date.issued | 1990 | en_US |
dc.identifier.citation | Lee, I. H., Meyerhoff, M. E. (1990)."Rapid flow-injection sandwich-type immunoassays of proteins using an immobilized antibody reactor and adenosine deaminase-antibody conjugates." Analytica Chimica Acta 229(): 47-55. <http://hdl.handle.net/2027.42/28783> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TF4-44Y3VXY-GP/2/3747b4434b65a7c613831d8b64366e16 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28783 | |
dc.description.abstract | A rapid flow-injection sandwich enzyme immunoassay suitable for the direct determination of proteins in biological samples is described. The proposed system utilizes highly active adenosine deaminase--antibody conjugates in conjunction with a flow-through immunoreactor and an ammonium ion-selective potentiometric detector. After appropriate sample/reagent injection steps, the enzyme activity bound to the reactor is measured by diverting a coninuously flowing stream of substrate (adenosine) through the packed immunocolumn and detecting liberated ammonium ions downstream with a tubular ammonium ion-selective electrode. The bound enzyme activity is directly proportional to the concentration of analyte in the original sample. By using non-equilibrium flow-rates of sample and reagent slugs, a single protein assay takes less than 12 min, including regeneration of the reactor. The proposed method is shown to be selective, reproducible and capable of determining accurately the model protein (human IgC) at sub-[mu]g ml-1 concentrations. | en_US |
dc.format.extent | 800275 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Rapid flow-injection sandwich-type immunoassays of proteins using an immobilized antibody reactor and adenosine deaminase-antibody conjugates | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055 U.S.A. | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055 U.S.A. | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28783/1/0000616.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0003-2670(00)85108-9 | en_US |
dc.identifier.source | Analytica Chimica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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