Differential regulation of smooth muscle contraction in rabbit internal anal sphincter by substance and bombesin
dc.contributor.author | Bitar, Khalil N. | en_US |
dc.contributor.author | Hillemeier, Craig | en_US |
dc.contributor.author | Biancani, Piero | en_US |
dc.date.accessioned | 2006-04-10T13:59:34Z | |
dc.date.available | 2006-04-10T13:59:34Z | |
dc.date.issued | 1990 | en_US |
dc.identifier.citation | Bitar, Khalil N., Hillemeier, Craig, Biancani, Piero (1990)."Differential regulation of smooth muscle contraction in rabbit internal anal sphincter by substance and bombesin." Life Sciences 47(26): 2429-2434. <http://hdl.handle.net/2027.42/28962> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T99-477974Y-CJ/2/5a37e1480067178ad96742aa124a16d9 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/28962 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1702175&dopt=citation | en_US |
dc.description.abstract | Substance P and bombesin induce contraction of isolated IAS smooth muscle cells by different intracellular mechanisms. The cells contracted in a dose dependent manner to both peptides. The kinetics of contraction were different. Substance P induced contraction peaked at 30 seconds and declined in a time dependent manner while bombesin induced contraction peaked at 30 seconds and was maintained for up to 8 minutes. The absence of extracellular calcium in the medium (0 calcium and 2 mM EGTA) had no affect on substance P induced contraction while it blocked bombesin induced contraction. Substance P induced contraction was blocked by the calmodulin antagonist W7 (10-9M) and was not affected by the PKC antagonist H7 (10-6M). Bombesin induced contraction was blocked by the PKC antagonist H7 and was not affected by the calmodulin antagonist W7. Our data indicate that substance P induces a transient contraction utilizing intracellular calcium and a calmodulin dependent pathway, while bombesin induces a sustained contraction utilizing calcium from extracellular sources and a calmodulin independent pathway. | en_US |
dc.format.extent | 395367 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Differential regulation of smooth muscle contraction in rabbit internal anal sphincter by substance and bombesin | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Pediatric Gastroenterology, University of Michigan Medical Center, Ann Arbor, MI 48109-0658, USA | en_US |
dc.contributor.affiliationum | Division of Pediatric Gastroenterology, University of Michigan Medical Center, Ann Arbor, MI 48109-0658, USA | en_US |
dc.contributor.affiliationother | Rhode Island Hospital, Brown University, Providence, RI, USA | en_US |
dc.identifier.pmid | 1702175 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/28962/1/0000799.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0024-3205(90)90487-C | en_US |
dc.identifier.source | Life Sciences | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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