Myosin light chain 1 isoform expression remains constant during ageing in Wistar F455 rats

Abstract

In order to study muscle gene expression during ageing, we examined both protein and total cellular RNA from Wistar F455 rat soleus and extensor digitorum longus (EDL) muscles at a variety of chronological ages. We found no evidence of the reappearance of the fast protein isoform of myosin light chain 1 [MLC1] in the slow soleus muscle during ageing previously reported by Syrovy and Gutmann, Pflugers Arch., 369 (1977) 85-89. We used both SDS-PAGE analysis of MLC1 proteins and slot blot RNA analysis with a probe specific for rat fast MLC1 mRNA (pC91), and found no changes in fast MLC1 expression during ageing in soleus or EDL muscles from these rats. These results indicate that re-expression of the fast MLC1 isoform is not a universal property of ageing soleus muscle.