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A simple method for the purification of human peripheral blood monocytes : A substitute for Sepracell-MN
dc.contributor.authorDenholm, Elizabeth M.en_US
dc.contributor.authorWolber, Frances M.en_US
dc.date.accessioned2006-04-10T14:30:43Z
dc.date.available2006-04-10T14:30:43Z
dc.date.issued1991-11-22en_US
dc.identifier.citationDenholm, Elizabeth M., Wolber, Frances M. (1991/11/22)."A simple method for the purification of human peripheral blood monocytes : A substitute for Sepracell-MN." Journal of Immunological Methods 144(2): 247-251. <http://hdl.handle.net/2027.42/29026>en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/B6T2Y-476KWJF-1XD/2/053837c438870962f2a140f11382d6afen_US
dc.identifier.urihttps://hdl.handle.net/2027.42/29026
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1960422&dopt=citationen_US
dc.description.abstractSepracell-MN has provided a simple, rapid means of isolating peripheral blood monocytes. However this product is no longer available. Consequentially we have developed a Percoll gradient wich matches Sepracell-MN in simplicity and yield of monocytes. Using this Percoll gradient, an average of 7 x 106 monocytes with a purity of 83% were obtained from 30-40 ml of blood. These monocytes were at least 97% viable and responded to chemotactic stimuli in comparable numbers to those prepared using Sepracell-MN.en_US
dc.format.extent371620 bytes
dc.format.extent3118 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherElsevieren_US
dc.titleA simple method for the purification of human peripheral blood monocytes : A substitute for Sepracell-MNen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pathology, University of Michigan Medical Center, Ann Arbor, MI, U.S.A.en_US
dc.contributor.affiliationotherDivision of Hematology, Albany Medical College, Albany, NY, U.S.A.en_US
dc.identifier.pmid1960422en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/29026/1/0000056.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1016/0022-1759(91)90092-Ten_US
dc.identifier.sourceJournal of Immunological Methodsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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