2,4,5-Trihydroxyphenylalanine (6-hydroxy-DOPA) displaces [3H]AMPA binding in rat striatum
dc.contributor.author | Cha, Jang-Ho J. | en_US |
dc.contributor.author | Dure, Leon S. IV | en_US |
dc.contributor.author | Sakurai, Sharin Y. | en_US |
dc.contributor.author | Penney, John B. | en_US |
dc.contributor.author | Young, Anne B. | en_US |
dc.date.accessioned | 2006-04-10T14:33:06Z | |
dc.date.available | 2006-04-10T14:33:06Z | |
dc.date.issued | 1991-10-28 | en_US |
dc.identifier.citation | Cha, Jang-Ho J., Dure, Leon S., Sakurai, Sharin Y., Penney, John B., Young, Anne B. (1991/10/28)."2,4,5-Trihydroxyphenylalanine (6-hydroxy-DOPA) displaces [3H]AMPA binding in rat striatum." Neuroscience Letters 132(1): 55-58. <http://hdl.handle.net/2027.42/29079> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T0G-482RVJ4-2X/2/ff870d15e3754277de173a3439c7d33f | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29079 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1664920&dopt=citation | en_US |
dc.description.abstract | Excitatory amino acid (EAA) receptor-mediated events have recently been implicated in dopaminergic mechanisms of neurotoxicity. 2,4,5-Trihydroxyphenylalanine (6-hydroxy-DOPA, TOPA), the ortho-hydroxylated derivative of the dopamine precursor 2,4-dihydroxyphenylalanine (-DOPA), has recently been reported to have neurotoxic properties which are blocked by CNQX, a specific antagonist of the AMPA class of (non-NMDA) EAA receptors. We report here that 6-hydroxy-DOPA is a selective displacer of [3H]AMPA binding in rodent brain. 6-Hydroxy-DOPA was as potent as kainate in displacing [3H]AMPA binding, with an IC50 value of 32 [mu]M. Ineffective displacers of [3H]AMPA binding included dopamine, 6-hydroxydopamine, -DOPA, -DOPA, carbidopa, DOPAC, [beta]-methylamino--alanine, 2,4-dihydroxyphenylacetyl--asparagine, homogentisic acid, 2,4-dihydroxyphenylacetic acid, amantadine, and threo-DOPS. 6-Hydroxy-DOPA (100 [mu]M) also displaced 20% of [3H]kainate binding, but did not displace binding to NMDA, phencyclidine (PCP), or dopaminergic (D1 and D2) receptors. These data raise the possibility that 6-hydroxy-DOPA or another abnormal metabolite of -DOPA could act as an excitotoxic agent via action at AMPA receptors. Given that non-NMDA receptors are postulated to play a role in neurotoxic events, these data provide an additional mechanism via which EAA receptor-mediated events could produce neurodegeneration in areas of brain with dopaminergic innervation. | en_US |
dc.format.extent | 331151 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | 2,4,5-Trihydroxyphenylalanine (6-hydroxy-DOPA) displaces [3H]AMPA binding in rat striatum | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 1664920 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29079/1/0000114.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0304-3940(91)90432-S | en_US |
dc.identifier.source | Neuroscience Letters | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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