Comparative anatomical distribution of 5-HT1A receptor mRNA and 5-HT1A binding in rat brain -- a combined in situ hybridisation/in vitro receptor autoradiographic study
dc.contributor.author | Chalmers, Derek T. | en_US |
dc.contributor.author | Watson, Stanley J. | en_US |
dc.date.accessioned | 2006-04-10T14:33:38Z | |
dc.date.available | 2006-04-10T14:33:38Z | |
dc.date.issued | 1991-10-04 | en_US |
dc.identifier.citation | Chalmers, D. T., Watson, S. J. (1991/10/04)."Comparative anatomical distribution of 5-HT1A receptor mRNA and 5-HT1A binding in rat brain -- a combined in situ hybridisation/in vitro receptor autoradiographic study." Brain Research 561(1): 51-60. <http://hdl.handle.net/2027.42/29092> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6SYR-4840MVD-17M/2/2554906f038605fc58c85293f0dbb379 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29092 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1797349&dopt=citation | en_US |
dc.description.abstract | The present study examined the comparative distribution of 5-HT1A receptor mRNA and 5-HT1A receptors in rat brain using a combination of in situ hybridisation histochemistry and in vitro receptor autoradiography. 5-HT1A mRNA was visualised using a 910 bp cRNA probe synthesised from a BalI-PvuII fragment of the rat 5-HT1A reetor gene, while 5-HT1A receptors were labelled with the 5-HT1A-selective ligand 8-OH-DPAT. In general terms, there was a complementary distribution of cells expressing 5-HT1A receptor mRNA and 5-HT1A receptor sites. High levels of both 5-HT1A mRNA and 5-HT1A receptors were evident in the hippocampal formation (CA1, CA3, dentate gyrus), entorhinal cortex, and raphe nuclei and lower levels in neocortex and thalamus. Although 5-HT1A mRNA was not expressed in any regions which did not also exhibit 5-HT1A receptors, within both the diagonal band and the medial septal nucleus mRNA levels were proportionately higher than 5-HT1A receptor levels, possibly reflecting receptor transport or a heterogeneity in 5-HT1A receptor turnover mechanisms. 5-HT1A receptor mRNA and 5-HT1A binding sites were undetectable in caudate/putamen and cerebellar regions. The present data indicate the synthesis of 5-HT1A receptors both in raphe serotonergic cells and anatomically specific serotonergic projection areas, further supporting both a presynaptic autoregulatory and postsynaptic modulatory role for this receptor in serotonergic transmission. | en_US |
dc.format.extent | 746760 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Comparative anatomical distribution of 5-HT1A receptor mRNA and 5-HT1A binding in rat brain -- a combined in situ hybridisation/in vitro receptor autoradiographic study | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.contributor.affiliationum | Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, U.S.A. | en_US |
dc.identifier.pmid | 1797349 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29092/1/0000127.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-8993(91)90748-K | en_US |
dc.identifier.source | Brain Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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