Differential expression of glycoproteins containing [alpha]--galactosyl groups on normal human breast epithelial cells and MCF-7 human breast carcinoma cells
dc.contributor.author | Petryniak, Jerzy | en_US |
dc.contributor.author | Varani, James | en_US |
dc.contributor.author | Ervin, Paul R. | en_US |
dc.contributor.author | Goldstein, Irwin J. | en_US |
dc.date.accessioned | 2006-04-10T14:33:40Z | |
dc.date.available | 2006-04-10T14:33:40Z | |
dc.date.issued | 1991-10 | en_US |
dc.identifier.citation | Petryniak, J., Varani, J., Ervin, P. R., Goldstein, I. J. (1991/10)."Differential expression of glycoproteins containing [alpha]--galactosyl groups on normal human breast epithelial cells and MCF-7 human breast carcinoma cells." Cancer Letters 60(1): 59-65. <http://hdl.handle.net/2027.42/29093> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T54-4BY9R7Y-1D6/2/9c666d969e4047347e7c0d16bb364067 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29093 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1913627&dopt=citation | en_US |
dc.description.abstract | Cell surface glycoproteins were isolated from the lysates of 125I-labeled normal human mammary epithelial cells (NHMEC) and from the human breast carcinoma cell line MCF-7, of blood-group O phenotype, by affinity chromatography on Griffonia simplicifolia I lectin-Sepharose. Specific elution of glycoproteins from the column with methyl [alpha]--galactoside suggests the presence of [alpha]--galactosyl groups on these moieties. SDS-PAGE analysis of isolated glycoproteins revealed both quantitative and qualitative differences between glycoproteins from normal and malignant cells. Three major glycoproteins of Mr 180 kDa, 85 kDa and the 44 kDa were obtained from MCF-7 cells. The 180-kDa glycoprotein was absent in NHMEC and the 44-kDa glycoprotein was very weakly expressed in these cells. The only glycoprotein which was found in almost equal amount in the lysate from both normal and malignant cells was the 85-kDa glycoprotein. These results indicate differences between normal human mammary epithelial cells and one kind of malignant human mammary epithelial cells, in the expression of glycoproteins containing [alpha]--galactosyl groups, irrespective of blood-group phenotype; they also demonstrate that [alpha]--galactosyl group are expressed in a very restrictive manner on the surface of this tumor cell line. | en_US |
dc.format.extent | 672758 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Differential expression of glycoproteins containing [alpha]--galactosyl groups on normal human breast epithelial cells and MCF-7 human breast carcinoma cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan, U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, U.S.A. | en_US |
dc.identifier.pmid | 1913627 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29093/1/0000129.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0304-3835(91)90049-N | en_US |
dc.identifier.source | Cancer Letters | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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