Mitochondrial protein p26 BCL2 reduces growth factor requirements of NIH3T3 fibroblasts
dc.contributor.author | Reed, John C. | en_US |
dc.contributor.author | Talwar, Harvinder S. | en_US |
dc.contributor.author | Cuddy, Michael | en_US |
dc.contributor.author | Baffy, Gyorgy | en_US |
dc.contributor.author | Williamson, John | en_US |
dc.contributor.author | Rapp, Ulf R. | en_US |
dc.contributor.author | Fisher, Gary J. | en_US |
dc.date.accessioned | 2006-04-10T14:37:53Z | |
dc.date.available | 2006-04-10T14:37:53Z | |
dc.date.issued | 1991-08 | en_US |
dc.identifier.citation | Reed, John C., Talwar, Harvinder S., Cuddy, Michael, Baffy, Gyorgy, Williamson, John, Rapp, Ulf R., Fisher, Gary J. (1991/08)."Mitochondrial protein p26 BCL2 reduces growth factor requirements of NIH3T3 fibroblasts." Experimental Cell Research 195(2): 277-283. <http://hdl.handle.net/2027.42/29195> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WFC-4DMNBX2-PN/2/c5ca67af74091bbd57e9e0e61151bc09 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29195 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2070813&dopt=citation | en_US |
dc.description.abstract | The BCL2 (B cell lymphoma/leukemia-2) proto-oncogene encodes a 26-kDa protein that has been localized to the inner mitochondrial membrane and that has been shown to enhance the survival of some types of hematopoietic cells. Here we show that NIH3T3 fibroblasts stably transfected with a BCL2 expression plasmid exhibit reduced dependence on competence-inducing growth factors (platelet-derived growth factor, PDGF; epidermal growth factor, EGF) for initiation of DNA synthesis. The importance of BCL2 for growth factorinduced proliferation of these cells was further confirmed by the useage of BCL2 antisense oligodeoxynucleotides. The mechanisms by which overexpression of p26 BCL2 contributes to fibroblast proliferation are unknown, but do not involve alterations in: (a) the production of inositol triphosphates (IP3), (b) PDGF-induced transient elevations in cytosolic Ca2+ ions, or (c) the activity of protein kinase C enzymes in these transfected cells. The results imply that changes in mitochondrial functions play an important role in the early stages of the cell cycle that render 3T3 cells competent to respond to the serum progression factors that stimulate entry into S-phase. | en_US |
dc.format.extent | 1224065 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Mitochondrial protein p26 BCL2 reduces growth factor requirements of NIH3T3 fibroblasts | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan School of Medicine, Department of Dermatology, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationum | University of Michigan School of Medicine, Department of Dermatology, Ann Arbor, Michigan 48109, U.S.A. | en_US |
dc.contributor.affiliationother | University of Pennsylvania School of Medicine, Department of Pathology and Laboratory Medicine, Philadelphia, Pennsylvania 19104, U.S.A. | en_US |
dc.contributor.affiliationother | University of Pennsylvania School of Medicine, Department of Pathology and Laboratory Medicine, Philadelphia, Pennsylvania 19104, U.S.A. | en_US |
dc.contributor.affiliationother | University of Pennsylvania School of Medicine, Department of Biochemistry, Philadelphia, Pennsylvania 19104, U.S.A. | en_US |
dc.contributor.affiliationother | University of Pennsylvania School of Medicine, Department of Biochemistry, Philadelphia, Pennsylvania 19104, U.S.A. | en_US |
dc.contributor.affiliationother | The National Cancer Institute Research Facility at Frederick, Laboratory of Viral Carcinogenesis, Frederick, Maryland 21710, U.S.A. | en_US |
dc.identifier.pmid | 2070813 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29195/1/0000249.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0014-4827(91)90374-4 | en_US |
dc.identifier.source | Experimental Cell Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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