Interaction of five -mannose-specific lectins with a series of synthetic branched trisaccharides
dc.contributor.author | Kaku, Hanae | en_US |
dc.contributor.author | Goldstein, Irwin J. | en_US |
dc.contributor.author | Oscarson, Stefan | en_US |
dc.date.accessioned | 2006-04-10T14:41:10Z | |
dc.date.available | 2006-04-10T14:41:10Z | |
dc.date.issued | 1991-06-25 | en_US |
dc.identifier.citation | Kaku, Hanae, Goldstein, Irwin J., Oscarson, Stefan (1991/06/25)."Interaction of five -mannose-specific lectins with a series of synthetic branched trisaccharides." Carbohydrate Research 213(): 109-116. <http://hdl.handle.net/2027.42/29275> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TFF-42WH3PD-1V/2/055201df186a208e33b952eb7afb653e | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29275 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1933932&dopt=citation | en_US |
dc.description.abstract | The interaction of a series of synthetic, branched trisaccharides with five -mannose-specific lectins was studied by precipitation-inhibition assay. The branched methyl [alpha]--mannotrioside, [alpha]--Manp-(1-->3)-[[alpha]--Manp-(1-->6)-[alpha]--ManpOMe, the best inhibitor of the Con A--Dextran interaction, was 42 times more potent than [alpha]--ManpOMe, and 3-6 times more potent than the two trisaccharides substituted with -glucosyl groups, and 8-15 times those with -galactosyl groups. Surprisingly, methyl O-[alpha]--mannopyranosyl-(1-->3)-[alpha]--mannopyranoside was bound to Con A 8-fold more avidly than methyl [alpha]--mannopyranoside. However, the related pea lectin (PSA) was singularly different from Con A in its carbohydrate-binding activity, showing no significantly enhanced binding to any of the sugars examined. The trisaccharides containing terminal, nonreducing, (1-->3)-linked [alpha]--mannopyranosyl groups, i.e., [alpha]--Manp-(1-->3)-[[alpha]--Glcp-(1-->6)-[alpha]--ManpOMe, [alpha]--Manp-(1-->3)-][alpha]--Galp-(1-->6)]-[alpha]--ManpOMe, and [alpha]--Manp-(1-->3)-[[alpha]--Manp-(1-->6)]-[alpha]-- ManpOMe, were the best inhibitors of the snowdrop lectin (GNA)--mannan precipitation system. On the other hand, all branched trisaccharides exhibited very similar inhibitory potencies toward the daffodil lectin (NPA)--mannan interaction, whereas [alpha]--Manp-(1-->3)-[[alpha]--Galp-(1-->6)]-[alpha]--ManpOMe and [alpha]--Manp-(1-->3)-[[alpha]--Manp-(1-->6)]-[alpha]--ManpOMe were somewhat better inhibitors than the other branched trisaccharides of the amaryllis lectin (HHA)--mannan precipitation reaction. Of the oligosaccharides studied, the linear trisaccharide [alpha]--Manp-(1-->6)-[alpha]--Manp-(1-->6)--Man appears to be the most complementary to the combining site(s) of NPA and HHA. | en_US |
dc.format.extent | 568189 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Interaction of five -mannose-specific lectins with a series of synthetic branched trisaccharides | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 U.S.A. | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109 U.S.A. | en_US |
dc.contributor.affiliationother | Department of Organic Chemistry, University of Stockholm, Stockholm S-109 91 Sweden | en_US |
dc.identifier.pmid | 1933932 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29275/1/0000334.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0008-6215(00)90602-5 | en_US |
dc.identifier.source | Carbohydrate Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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