Molecular cloning of porcine [alpha]1-microglobulin/HI-30 reveals developmental and tissue-specific expression of two variant messenger ribonucleic acids
dc.contributor.author | Tavakkol, Amir | en_US |
dc.date.accessioned | 2006-04-10T14:50:11Z | |
dc.date.available | 2006-04-10T14:50:11Z | |
dc.date.issued | 1991-01-17 | en_US |
dc.identifier.citation | Tavakkol, Amir (1991/01/17)."Molecular cloning of porcine [alpha]1-microglobulin/HI-30 reveals developmental and tissue-specific expression of two variant messenger ribonucleic acids." Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 1088(1): 47-56. <http://hdl.handle.net/2027.42/29501> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T1V-486TCG8-4H/2/080525f2b996e95012914c6d2a184a57 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29501 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1703444&dopt=citation | en_US |
dc.description.abstract | A 1008 basepair (bp) cDNA clone encoding 335 amino acids followed by an inframe TGA translation termination codon and a 295-nucleotide 3' untranslated (UT) region has been isolated from a pig liver cDNA library. Based on the deduced amino acid and nucleotide sequence homology to a human cDNA (Kaumeyer, J.F., Polazzi, J.O. and Kotick, M.P. (1986) Nucleic Acids Res. 14, 7839-7850), the 5' amino terminus was found to code for [alpha]1-microglobulin ([alpha]1-M), a 183 amino acid protein belonging to the lipocalin protein superfamily (Pervaiz, S. and Brew, K. (1985) Science 228, 335-337). The 3' half encoded HI-30 which constitutes the Kunitz-type proteinase inhibitory (L-chain) domain of porcine inter-alpha-trypsin inhibitor (I[alpha]TI). In Northern blot hybridization, this cDNA identified two equally abundant mRNA species of approx. 1.3 kb and 1.6 kb in length. However, a 125 bp cDNA probe derived from the 3' UT region of the cDNA hybridized only to the 1.6 kb mRNA. The differences observed in the 3' UT region of these mRNAs suggest the utilization of alternative polyadenylation signals or presence of unprocessed nuclear RNA. Densitometric scanning of Northern blots indicated that [alpha]1-M/HI-30 mRNA levels were higher (5-8-fold) in fetal and neonatal liver compared to that of primiparous pigs. In contrast, the RNA levels did not change significantly during pregnancy. Dot blot analysis of RNA indicated liver to be the major site of [alpha]1-M/HI-30 mRNA expression with lower levels observed in the stomach. The results suggest that modulation of [alpha]1-M/HI-30 gene expression could play a role during porcine growth. Increased I[alpha]Tl L-chain mRNA levels may be particularly important in fetal and neonatal development when regulation of the inflammatory response and protection of macromolecules from proteolytic degradation is vital to survival and sustained growth. | en_US |
dc.format.extent | 852039 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Molecular cloning of porcine [alpha]1-microglobulin/HI-30 reveals developmental and tissue-specific expression of two variant messenger ribonucleic acids | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Dermatology, The University of Michigan, Ann Arbor, MI, U.S.A. | en_US |
dc.identifier.pmid | 1703444 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29501/1/0000587.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0167-4781(91)90152-C | en_US |
dc.identifier.source | Biochimica et Biophysica Acta | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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