Kinetics of TNF, IL-6, and IL-8 gene expression in LPS-stimulated human whole blood
dc.contributor.author | DeForge, Laura E. | en_US |
dc.contributor.author | Remick, Daniel G. | en_US |
dc.date.accessioned | 2006-04-10T14:50:16Z | |
dc.date.available | 2006-04-10T14:50:16Z | |
dc.date.issued | 1991-01-15 | en_US |
dc.identifier.citation | DeForge, L. E., Remick, D. G. (1991/01/15)."Kinetics of TNF, IL-6, and IL-8 gene expression in LPS-stimulated human whole blood." Biochemical and Biophysical Research Communications 174(1): 18-24. <http://hdl.handle.net/2027.42/29503> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WBK-4DN92WT-72/2/0fc98abe7e2f5097733f12ec4b222977 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29503 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1989598&dopt=citation | en_US |
dc.description.abstract | While the production of tumor necrosis factor (TNF) and interleukin-6 (IL-6) in septic shock and other inflammatory states is well established, the role of interleukin-8 (IL-8), a recently described neutrophil chemoattractant and activator, has yet to be fully elucidated. Using lipopolysaccharide (LPS)-stimulated human whole blood as an ex vivo model of sepsis, the kinetics of messenger RNA (mRNA) up-regulation and protein release of these cytokines were examined. Two waves of cytokine gene activation were documented. TNF and IL-6 were induced in the first wave with mRNA levels peaking between 2-4 hours and then rapidly declining. TNF and IL-6 protein peaked at 4-6 hours and then stabilized. IL-8 mRNA and protein were induced in the first wave, reached a plateau between 6-12 hours, and rose again in a second wave which continued to escalate until the end of the 24 hour study. These data demonstrate the complex patterns of cytokine gene expression and suggest that production of early mediators may augment continued expression of IL-8 to recruit and retain neutrophils at a site of inflammation. | en_US |
dc.format.extent | 710655 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Kinetics of TNF, IL-6, and IL-8 gene expression in LPS-stimulated human whole blood | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-0602, USA | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-0602, USA | en_US |
dc.identifier.pmid | 1989598 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29503/1/0000589.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0006-291X(91)90478-P | en_US |
dc.identifier.source | Biochemical and Biophysical Research Communications | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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