Comparison of the isolation of adducts fo 2'-deoxycytidine and 2'-deoxyguanosine with phenylglycidyl ether by high-performance liquid chromatography on a reversed-phase column and a polystyrene-divinylbenzene column
dc.contributor.author | Van Den Eeckhout, E. | en_US |
dc.contributor.author | Coene, J. | en_US |
dc.contributor.author | Claereboudt, J. | en_US |
dc.contributor.author | Borremans, F. | en_US |
dc.contributor.author | Claeys, M. | en_US |
dc.contributor.author | Esmans, E. L. | en_US |
dc.contributor.author | Sinsheimer, Joseph E. | en_US |
dc.date.accessioned | 2006-04-10T14:54:10Z | |
dc.date.available | 2006-04-10T14:54:10Z | |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | van den Eeckhout, E., Coene, J., Claereboudt, J., Borremans, F., Claeys, M., Esmans, E., Sinsheimer, J. E. (1991)."Comparison of the isolation of adducts fo 2'-deoxycytidine and 2'-deoxyguanosine with phenylglycidyl ether by high-performance liquid chromatography on a reversed-phase column and a polystyrene-divinylbenzene column." Journal of Chromatography A 541(): 317-331. <http://hdl.handle.net/2027.42/29601> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6TG8-44TP5BC-4V/2/e93311e4a87cb95e41100f19fb1d6acd | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29601 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2037652&dopt=citation | en_US |
dc.description.abstract | 2'-Deoxycitidine (dCyd) and 2'-deoxyguanosine (dGuo) were subjected to reaction with phenylglycidyl ether (PGE) in methanol in order to study the formation of the corresponding 2'-deoxynucleoside adducts. Separation methods were developed on analytical and semi-preparative scales using high-performance liquid chromatography with photodiode-array detection on a reversed-phase column and on a polystyrene-divinylbenzene column. The use of the latter column was prompted by decomposition of the preparatively isolated dGuo-PGE adducts on the reversed-phase column. The use of a polystyrene-divinylbenzene column solved this problem and also revealed the presence of one more peak in both the dCyd-and dGuo-PGE reaction mixtures.The adducts of dCyd and dGuo were isolated on preparative reversed-phase and polystyrene-divinylbenzene columns and characterized by UV, fast atom bombardment mass and 360 MHz 1H NMR spectrometry. The adducts of dCyd were the diastereomers of N-3-(2-hydroxy-3-phenoxypropyl)-2'-deoxycytidine and N4-(2-hydroxy-3-phenoxypropyl)-2'-deoxycytidine whereas those of dGuo were the two diastereomers of N-7-(2-hydroxy-3-phenoxypropyl)-2'-deoxyguanosine and a third peak which appeared to be mainly (N2-(2-hydroxy-3-phenoxypropyl)-2'-deoxyguanosine. | en_US |
dc.format.extent | 935791 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Comparison of the isolation of adducts fo 2'-deoxycytidine and 2'-deoxyguanosine with phenylglycidyl ether by high-performance liquid chromatography on a reversed-phase column and a polystyrene-divinylbenzene column | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | College of Pharmacy, University of Michigan, Ann Arbor, MI 48109 U.S.A. | en_US |
dc.contributor.affiliationother | College of Pharmacy, University of Ghent, Harelbekestraat 72, B-9000 Ghent Belgium | en_US |
dc.contributor.affiliationother | College of Pharmacy, University of Ghent, Harelbekestraat 72, B-9000 Ghent Belgium | en_US |
dc.contributor.affiliationother | Department of Pharmacy, University of Antwerp (U.I.A.), Universiteitsplein 1, B-2610 Wilrijk Belgium | en_US |
dc.contributor.affiliationother | Laboratory for Organic Chemistry, University of Ghent, Krijgslaan 281, B-9000 Ghent Belgium | en_US |
dc.contributor.affiliationother | Department of Pharmacy, University of Antwerp (U.I.A.), Universiteitsplein 1, B-2610 Wilrijk Belgium | en_US |
dc.contributor.affiliationother | Laboratory for Organic Chemistry, University of Antwerp (R.U.C.A.), Groenenborgerlaan 171, B-2020 Antwerp Belgium | en_US |
dc.identifier.pmid | 2037652 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29601/1/0000690.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0021-9673(01)96003-0 | en_US |
dc.identifier.source | Journal of Chromatography A | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.