A radiation hybrid map of the proximal short arm of the human X chromosome spanning incontinentia pigmenti 1 (IP1) translocation breakpoints
dc.contributor.author | Gorski, Jerome L. | en_US |
dc.contributor.author | Boehnke, Michael | en_US |
dc.contributor.author | Reyner, Eric L. | en_US |
dc.contributor.author | Burright, Eric N. | en_US |
dc.date.accessioned | 2006-04-10T15:01:30Z | |
dc.date.available | 2006-04-10T15:01:30Z | |
dc.date.issued | 1992-11 | en_US |
dc.identifier.citation | Gorski, Jerome L., Boehnke, Michael, Reyner, Eric L., Burright, Eric N. (1992/11)."A radiation hybrid map of the proximal short arm of the human X chromosome spanning incontinentia pigmenti 1 (IP1) translocation breakpoints." Genomics 14(3): 657-665. <http://hdl.handle.net/2027.42/29766> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WG1-4GSCHKG-H/2/47e4d92b560c3886179756cb961fa931 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29766 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1427892&dopt=citation | en_US |
dc.description.abstract | Radiation hybrid mapping was used in combination with physical mapping techniques to order and estimate distances between 14 loci in the proximal region of the short arm of the human X chromosome. A panel of radiation hybrids containing human X-chromosomal fragments was generated from a Chinese hamster-human cell hybrid containing an X chromosome as its only human DNA. Sixty-seven radiation hybrids were screened by Southern hybridization with sets of probes that mapped to the region Xp11.4-Xcen to generate a radiation hybrid map of the area. A physical map of 14 loci was constructed based on the segregation of the loci in the hybrid clones. Using pulsed-field gel electrophoresis (PFGE) analyses and a somatic cell hybrid mapping panel containing naturally occurring X; autosome translocations, the order of the 14 loci was verified and the loci nearest to the X-chromosomal translocation breakpoints associated with the disease incontinentia pigmenti 1 (IP1) were identified. The radiation hybrid panel will be useful as a mapping resource for determining the location, order, and distances between other genes and polymorphic loci in this region as well as for generating additional region-specific DNA markers. | en_US |
dc.format.extent | 1367081 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | A radiation hybrid map of the proximal short arm of the human X chromosome spanning incontinentia pigmenti 1 (IP1) translocation breakpoints | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109-0688, USA; Department of Human Genetics, University of Michigan, Ann Arbor, Michigan 48109-0688, USA. | en_US |
dc.contributor.affiliationum | Department of Biostatistics, University of Michigan, Ann Arbor, Michigan 48109-0688, USA | en_US |
dc.contributor.affiliationum | Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109-0688, USA | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan, Ann Arbor, Michigan 48109-0688, USA | en_US |
dc.identifier.pmid | 1427892 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29766/1/0000104.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0888-7543(05)80165-X | en_US |
dc.identifier.source | Genomics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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