Rapid generation of region-specific genomic clones by chromosome microdissection: Isolation of DNA from a region frequently deleted in malignant melanoma
dc.contributor.author | Guan, Xin-Yuan | en_US |
dc.contributor.author | Meltzer, Paul S. | en_US |
dc.contributor.author | Cao, Jun | en_US |
dc.contributor.author | Trent, Jeffrey M. | en_US |
dc.date.accessioned | 2006-04-10T15:01:33Z | |
dc.date.available | 2006-04-10T15:01:33Z | |
dc.date.issued | 1992-11 | en_US |
dc.identifier.citation | Guan, Xin-Yuan, Meltzer, Paul S., Cao, Jun, Trent, Jeffrey M. (1992/11)."Rapid generation of region-specific genomic clones by chromosome microdissection: Isolation of DNA from a region frequently deleted in malignant melanoma." Genomics 14(3): 680-684. <http://hdl.handle.net/2027.42/29767> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6WG1-4GSCHKG-M/2/035fceaca740ffe2c57426859fc75d3c | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29767 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1427895&dopt=citation | en_US |
dc.description.abstract | Malignant melanoma is frequently characterized by the deletion of the long arm of chromosome 6 (usually encompassing 6q16-q21). In an effort to saturate this region with DNA markers, microdissection and molecular cloning of DNA from banded human metaphases have been performed. This work was facilitated by the recent development of a novel chromosome microdissection scheme that omits microchemical manipulation of DNA. Microdissection was targeted on band 6q21. Direct PCR amplification of dissected DNA was first used as a probe in chromosomal in situ hybridization of normal metaphases to confirm the specificity of material excised for cloning. A genomic library of 20,000 clones, which is highly enriched for sequences encompassing 6q21, was then constructed. Clones from this library have been mapped against a human-rodent somatic cell hybrid mapping panel that divides chromosome 6 into seven regions, confirming the localization of probes within the target region. Direct PCR amplification of DNA excised by microdissection greatly simplifies and facilitates this chromosome band-specific cloning strategy. The isolation of microclones from this region of chromosome 6 should assist in establishing a physical map of the melanoma deletion region. | en_US |
dc.format.extent | 2662299 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Rapid generation of region-specific genomic clones by chromosome microdissection: Isolation of DNA from a region frequently deleted in malignant melanoma | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pediatrics, The University of Michigan Comprehensive Cancer Center, The University of Michigan Medical Center, MSRB II C560, 1150 West Medical Center Drive, Ann Arbor, Michigan 48109-0668, USA | en_US |
dc.contributor.affiliationum | Department of Radiation Oncology, The University of Michigan Comprehensive Cancer Center, The University of Michigan Medical Center, MSRB II C560, 1150 West Medical Center Drive, Ann Arbor, Michigan 48109-0668, USA | en_US |
dc.contributor.affiliationum | Department of Pediatrics, The University of Michigan Comprehensive Cancer Center, The University of Michigan Medical Center, MSRB II C560, 1150 West Medical Center Drive, Ann Arbor, Michigan 48109-0668, USA; Department of Human Genetics, The University of Michigan Comprehensive Cancer Center, The University of Michigan Medical Center, MSRB II C560, 1150 West Medical Center Drive, Ann Arbor, Michigan 48109-0668, USA. | en_US |
dc.contributor.affiliationother | Committee on Genetics, University of Arizona, Tucson, Arizona 85724, USA | en_US |
dc.identifier.pmid | 1427895 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29767/1/0000105.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/S0888-7543(05)80168-5 | en_US |
dc.identifier.source | Genomics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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