Gene expression of the insulin-like growth factors and their receptors in human neuroblastoma cell lines
dc.contributor.author | Martin, Donna M. | en_US |
dc.contributor.author | Yee, Douglas | en_US |
dc.contributor.author | Carlson, Robert O. | en_US |
dc.contributor.author | Feldman, Eva L. | en_US |
dc.date.accessioned | 2006-04-10T15:03:17Z | |
dc.date.available | 2006-04-10T15:03:17Z | |
dc.date.issued | 1992-10 | en_US |
dc.identifier.citation | Martin, D. M., Yee, D., Carlson, R. O., Feldman, E. L. (1992/10)."Gene expression of the insulin-like growth factors and their receptors in human neuroblastoma cell lines." Molecular Brain Research 15(3-4): 241-246. <http://hdl.handle.net/2027.42/29806> | en_US |
dc.identifier.uri | http://www.sciencedirect.com/science/article/B6T07-4859N3B-6B/2/55031b14f84bd9e2503dcab549ea5025 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/29806 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1331680&dopt=citation | en_US |
dc.description.abstract | Insulin-like growth factors (IGF) I and II are polypeptides with both growth-promoting and insulin-like metabolic effects. The developmentally specific expression of IGF I and II in the nervous system implies a role for these growth factors in neuronal growth and differentiation. In the present study, we analyzed IGF and IGF receptor mRNA transcripts from two related human neuroblastoma cell lines, SH-SY5Y and SK-N-SH. These cell lines provide a good in vitro model of neuronal development. Northern analysis of total RNA from each cell line revealed three IGF II mRNA transcripts (6.0, 4.8, and 1.8 kb), and one mRNA transcript each for the type I (11.0 kb) and type II (9.4 kb) IGF receptors. The size distribution of these multiple transcripts is similar to that found during normal human fetal development. These results establish both cell lines as good in vitro models for investigating the mechanisms which underly IGF gene expression during nervous system development. | en_US |
dc.format.extent | 639443 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.title | Gene expression of the insulin-like growth factors and their receptors in human neuroblastoma cell lines | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Neurology and the Neuroscience Program, University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | The Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationum | Department of Neurology and the Neuroscience Program, University of Michigan, Ann Arbor, MI 48109, USA | en_US |
dc.contributor.affiliationother | Department of Medicine, Division of Oncology, University of Texas Health Science Center, San Antonio, TX 78284, USA | en_US |
dc.identifier.pmid | 1331680 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/29806/1/0000152.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/0169-328X(92)90114-Q | en_US |
dc.identifier.source | Molecular Brain Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.